ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Microbore columns
;
Column-switching
;
Myristicin
;
Serum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary A microbore high-performance liquid chromatographic method with column-switching was developed for the analysis of myristicin from rat serum without prepurification. Deproteinization, fractionation, concentration and separation of analyte were carried out by appropriate switching of columns and using solvent mixtures. The method showed excellent precision, accuracy and speed with a detection limit of 10 ng mL−1 from 25 μL of serum. The total analysis time per sample was 25 min and the coefficients of variation for intra- and inter-assay were less than 1.8%.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02467705
Permalink