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Separation of intramolecular NOE and exchange peaks in water exchange spectroscopy using spin-echo filters (1996)

Mori, Susumu ; Berg, Jeremy M. ; Zijl, Peter C. M.

Springer

Journal of biomolecular NMR 7 (1996), S. 77-82

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ISSN: 1573-5001

Keywords: Water exchange ; NOE ; Pulsed field gradients ; Macromolecules

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Chemistry and Pharmacology

Notes: Summary A technique for separating intramolecular NOE and solvent-proton exchange peaks in exchange spectroscopy is demonstrated. This method utilizes the large differences in relaxation and coupling properties of water and macromolecules to separate the two effects. The spin-echo filter consists of a water-frequency selective 90° pulse followed by a spin-echo sequence. If the echo time is sufficiently long, protein resonances (e.g. CαH protons) excited by the selective pulse are removed due to their much shorter T2 values and J-coupling evolution. By combining the filter with exchange spectroscopy (EXSY) or water exchange (WEX) filter experiments, exchange peaks can be selectively observed. In this paper the filter is combined with a modified version of the WEX filter (WEX II filter) with 1D and 2D detection and applied to a zinc finger peptide and to staphylococcal nuclease, allowing estimation of the contribution of intramolecular NOEs to the exchange spectra.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00190459

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Redesigning the DNA-binding specificity of a zinc finger protein: A data base-guided approach (1992)

Desjarlais, John R. ; Berg, Jeremy M.

New York, NY : Wiley-Blackwell

Proteins: Structure, Function, and Genetics 12 (1992), S. 101-104

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ISSN: 0887-3585

Keywords: protein-DNA interactions ; hydrogen bonding ; Sp1 ; recognition code ; amino acid correlations ; protein-DNA interface ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Medicine

Notes: A peptide corresponding to the three zinc finger domains of the human transcription factor Sp1 has been expressed and found to bind a consensus Sp1 binding site with the sequence 5′-GGGGCGGGG-3′. Examination of the amino acid distributions within a large zinc finger sequence data base and chemical arguments suggested that a particular Arg to Gln sequence change might convert binding specificity to 5′-GGGGCAGGG-3′. Experimental tests of this hypothesis revealed that such a change could be induced only when two other sequence changes, deduced from examination of sequence correlations, were made as well. These results provide the most direct information to date about how zinc finger proteins might recognize adenine-containing binding sites and bear on the existence and nature of any code between zinc finger protein and binding site sequences.

Additional Material: 4 Ill.