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  • 1
    ISSN: 1617-4623
    Keywords: Key words Differential display ; Heterosis ; RNA-binding protein ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A hybrid-specific expressed cDNA fragment, designated as AG5, has been identified in wheat seedling leaves using differential mRNA display. AG5 contains an open reading frame (ORF) encoding 183 amino acid residues. Comparison with amino acid sequences in GenBank revealed that the AG5 protein is homologous to a group of Gly-rich proteins with consensus sequence-type RNA-binding domains (CS-RBD). Structural analysis showed that AG5 protein contains five motifs, including a consensus sequence-type RNA-binding domain near its N-terminus, arginine/aspartic acid repeats and a Gly-rich region in its center, a Cys-X2-Cys-X4-His-X4-Cys (CCHC) zinc finger motif in the Gly-rich region, and TrySer2ArgAsp2Arg repeats towards its C-terminus. Of all previously described RNA-binding proteins, only RZ-1 from tobacco has a similar structure to the AG5 protein, but RZ-1 lacks a TrySer2ArgAsp2Arg repeat motif, indicating that the two proteins may belong to a family of closely related proteins in plants. The possible role of AG5 and its relation to wheat heterosis are discussed.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1439-5444
    Keywords: Key wordsLasius spp. ; Temporary parasitic ant ; Naturally mixed colony ; Nestmate recognition ; Cuticular profiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Workers of the temporary parasitic ant Lasius sp. were perfectly compatible with their hosts Lasius fuliginosus. Aggression was never seen between allospecific nestmates in either field or laboratory. In the laboratory, trophallaxis and allogrooming between allospecific nestmates were statistically more frequent than that between conspecifics. These ants were highly aggressive toward individuals of either species from another mixed colony located 1 km away, indicating that they discriminated nestmates and nonnestmates regardless of the species to which they belonged. No aggressive actions, however, were observed between two neighboring mixed nests located 5 m apart. We have not considered, however, the relationship of the two neighboring nests: the two nests may have been two subunits of the same family or sister colonies, or may have been different colonies but discriminated from distant aliens. Gas chromatography analysis on the total cuticular extracts showed that Lasius sp. and L. fuliginosus possess distinct cuticular profiles, even though they were collected from the same mixed colony.
    Type of Medium: Electronic Resource
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