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Digitale Medien

Measurement of equilin and estrone in human plasma by capillary gas chromatography-negative ion chemical ionization mass spectrometry (1987)

Robinson, P. R. ; Jones, M. D. ; Maddock, J.

Weinheim : Wiley-Blackwell

Journal of High Resolution Chromatography 10 (1987), S. 6-11

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ISSN: 0935-6304

Schlagwort(e): Capillary gas chromatography ; Negative ion mass spectrometry ; Plasma ; Estrone ; equilin ; Conjugated estrogens ; Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: A procedure is described for the analysis of the estrogens equilin and estrone in human plasma following oral administration of conjugated estrogen preparations. After enzymatic hydrolysis of the sulfate conjugates, plasma proteins are precipitated with methanol and the estrogens extracted into ethyl acetate. Derivatization with the reagent flophemesylamine converts equilin and estrone into volatile pentafluorophenyldimethylsilyl ethers ideally suited to capillary gas chromatography-negative ion chemical ionization mass spectrometry. Using a 15 meter dimethyl silicone bonded phase fused silica capillary column separation of the estrone and equilin derivatives is achieved within 9 minutes. Selected ion monitoring of the intense negative molecular ions enables levels of 1 ng.ml-1 to be measured with coefficients of variation of 9.3 % and 14.2 % for estrone and equilin respectively. Plasma levels of the compounds are reported in two male volunteers up to 24 hours after dosing with 5 milligrams of Premarin™. (™ Ayerst Laboratories Inc., New York, USA.).

Zusätzliches Material: 7 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jhrc.1240100102

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Digitale Medien

Poor results of distal trapezium resection for trapeziometacarpal-1 osteoarthritis (2000)

Damen, A. ; van der Lei, B. ; Schepel, S. ; [weitere]

Springer

European journal of plastic surgery 23 (2000), S. 227-231

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ISSN: 1435-0130

Schlagwort(e): Key words Trapeziometacarpal-1 ; Osteoarthritis ; Distal trapezium resection ; Failure

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Abstract Eleven patients (mean age 54 years, range 46–67 years) with osteoarthritis limited to the trapeziometacarpal-1 joint underwent a distal trapezium resection. In nine cases the palmaris longus tendon and in two cases a Swanson condylar implant was used as interposition material. The results were very disappointing. Only one patient was satisfied. Nine patients were reoperated upon (mean interval period 43 months, range 3–110) because of pain. In two cases, the cause was dislocation of the Swanson condylar implant. In the other seven cases, prominent bone on the trapezium, ulnar osteophytes, scaphotrapezial osteoarthritis, sclerosis of the trapezium remnant, and impingement were causal factors. In future, the selection of patients for this operation will be performed with even greater care. Despite these poor results, a definitive conclusion about the indication for this procedure can only be made after evaluation of an improved operative procedure.