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  • 1
    ISSN: 1432-041X
    Keywords: Transdifferentiation ; Transdetermination ; Cellular metaplasia ; Neural retina ; Lens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To elucidate the cell-type origin of lens cells, which differentiate in stationary cultures of neural retina, chimeric cultures between chick and quail cells were made to recombine xenoplastically the different cell fractions separated from 8- to 9-day cultures of 3.5-day-old embryonic neural retinal cells by means of centrifugation in Percoll. Extensive lentoidogenesis occurred in the recombination of the N2-fraction (consisting mostly of small round cells) with the E-fraction (containing a number of flattened epithelial cells). Taking advantage of the difference in electrophoretic mobility of chick and quail δ-crystallin, it was shown that this lens-specific protein, synthesized in the chimeric cultures, was mostly of the species-specificity of N2. Microscopic observations of histological sections of cell sheets of quail N2- and chick E-fraction chimeric cultures revealed that most cells with δ-crystallin, as identified by means of immunohistological detection, are provided with a nuclear marker characteristic of quail. By determining the level of activity of choline acetyltransferase and by examining the stainability with a fluorescent dye (Merocyanine-540), it was suggested that cells in the N2-fraction are primitive neuroblast-like cells. Thus, we can conclude that putative neuronal cells in early cultures of avian embryonic neural retina can transdifferentiate into lens cells.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Developmental Dynamics 196 (1993), S. 273-275 
    ISSN: 1058-8388
    Keywords: Retinal pigmented epithelial cells ; Lens ; Neural retina ; Crystallin genes ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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