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Activity and specificity of covalently immobolized wheat germ agglutinin toward cell surfaces (1973)

Zabriskie, Dane ; Ollis, David F. ; Burger, Max M.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 15 (1973), S. 981-992

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ISSN: 0006-3592

Keywords: Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Wheat germ agglutinin protein, which is able to agglutinate tumor cells better than normal cells, was covalently bound to polyacrylamide gel beads. The specific binding activity of the protein was preserved on these beads and was expressed heterogeneously by the binding of mouse leukemia cells (L1210) to the protein coupled gels. The selective activity of the immobilized protein was maximal when the number of sites available to covalently couple the protein was lowest. The application of this observation to the general field of covalent immobilization of proteins and enzymes may be of considerable utility.

Additional Material: 3 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/bit.260150515

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Hereditary motor endplate disease (med) of the mouse: Observations on dissociated myogenic cells and their development in culture (1980)

Jockusch, Harald ; Burkart, Werner ; Burger, Max M.

Springer

Cell & tissue research 207 (1980), S. 241-248

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ISSN: 1432-0878

Keywords: Neuromuscular disease ; Muscle culture ; Tissue dissociation ; Immunofluorescence

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary Myogenic cells from mice homozygous for the lethal mutation “motor endplate disease” (med/med) were grown in culture. Like muscle cells taken from wild type (+/?) litter mates they fused to form myotubes which contracted, developed cross striations, and exposed acetylcholine receptors (AChR) on their surface. However, a decrease of 30% in the number of mononucleated cells per unit fresh weight of muscle was observed as early as 2–3 days postnatal, i.e., at least one week prior to the onset of physiological symptoms. Hence, in addition to influencing the functional maintenance of motor endplates, the med gene seems to control early events in muscle development.