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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 24 (1993), S. 394-399 
    ISSN: 1432-0983
    Keywords: Neurospora crassa ; bli-7, eas, ccg-2 ; Promoter analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Expression of the Neurospora crassa gene bli-7, (identical with eas, and ccg-2), is induced by blue light, as well as glucose- or nitrogen-starvation. A promoter analysis was performed by an assay that does not involve promoter-reporter constructs but rather the insertion of foreign DNA into the transcribed sequence. To detect regulatory elements a series of deletions in the upstream region was generated. The inducibility of the gene, in response to the three inducing conditions mentioned, is lost by eliminating the region between-1498 bp and-1017 bp upstream of the transcription start point. A segment with an apparently negative effect was found between-595 bp and-429 bp, as well as a stretch of DNA from-429 bp to-380 bp which may exert a positive influence after light induction.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Planta 134 (1977), S. 61-66 
    ISSN: 1432-2048
    Keywords: Autochemotropism ; Avoidance response ; Ethylene ; Phycomyces
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The sporangiophore of the fungus Phycomyces blakesleeanus has the property of growing away from a barrier which is few mm from the growing zone of the sporangiophore (avoidance or autochemotropic response). A model has been published (Cohen, R.J., Jan, N.Y., Matricon, J., Delbrück, M.: J. Gen. Physiol. 66, 67–95 (1975)). To explain the avoidance response which postulates that the sporangiophore emits and readsorbs a volatile growth-promoting effector (gas X) and that the barrier modifies the effector distribution by acting as an aerodynamic obstacle, causing a higher concentration of gas X on the side of the sporangiophore closer to the barrier. From this model we deduced three properties of the gas X. Of the several gases tested (N2, CO2, CH4, C2H2, C2H4, C2H6) only ethylene (C2H4) had all these three properties, a finding which suggests that it has a role in the avoidance response (autochemotropism).
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1617-4623
    Keywords: Neurospora crassa ; Blue light ; Mitochondria ; Short-chain alcohol dehydrogenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Blue light plays an important role in developmental control throughout nature. Thebli-4 gene ofNeurospora crassa, together withbli-3, al-1 andal-2, is rapidly inducible by blue light. Induction leads to a ninety-fold increase in transcription rate over the dark control level, and the gene therefore appears to be of prime importance in the blue-light induction pathway ofN. crassa. We describe the sequencing and analysis ofbli-4 and the 38 kDa protein it encodes. We show that the protein is very rapidly imported into the mitochondria and exhibits high homology with the family of short-chain alcohol dehydrogenases.
    Type of Medium: Electronic Resource
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