Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    The location of (1→3)-β-glucans in the walls of pollen tubes of Nicotiana alata using a (1→3)-β-glucan-specific monoclonal antibody (1991)
    Springer
    Planta 185 (1991), S. 1-8 
    Details
    ISSN: 1432-2048
    Keywords: Callose ; Cell wall ; (1→3)-β-Glucan ; Nicotiana alata ; Pollen tube ; Monoclonal antibody
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The location of the (1→3)-β-glucan, callose, in the walls of pollen tubes in the style of Nicotiana alata Link et Otto was studied using specific monoclonal antibodies. The antibodies were raised against a laminarinhaemocyanin conjugate. One antibody selected for further characterization was specific for (1→3)-β-glucans and showed no binding activity against either a cellopentaose-bovine serum albumin (BSA) conjugate or a (1→3, 1→4)-β-glucan-BSA conjugate. Binding was inhibited by (1→3)-β-oligoglucosides (DP, 3–6) with maximum competition being shown by laminaripentaose and laminarihexaose, indicating that the epitope included at least five (1→3)-β-linked glucopyranose residues. The monoclonal antibody was determined to have an affinity constant for laminarihexaose of 2.7. 104M−1. When used with a second-stage gold-labelled, rabbit anti-mouse antibody, the monoclonal antibody probe specifically located the (1→3)-β-glucan in the inner wall layer of thin sections of the N. alata pollen tubes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194507
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    A nuclear sequence associated with self-incompatibility in Nicotiana alata has homology with mitochondrial DNA (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 320-324 
    Details
    ISSN: 1432-2242
    Keywords: Nicotiana alata ; Self-incompatibility ; Plant mitochondrial DNA ; Interorganeller sequence transfer ; Lycopersicon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A 1.0-kb nuclear fragment located 5′ to a coding sequence associated with self-incompatibility in N. alata shows homology with mitochondrial chromosomal DNA on Southern blots. This sequence is also present in the mitochondrial DNA of two species of tomato, L. esculentum and L. pennellii, but shows no homology to mtDNA of Zea mays. The homologous mitochondrial fragment from N. alata was cloned and sequenced. A short region of 56 bp matches the nuclear sequence in 53/56 bp. Other matched but misaligned segments flank the 3′ end. The nuclear sequence is marked at the 5′ end by two 8 bp direct repeats. The function of the nuclear sequence is not known although, it is located 397 bp upstream from the site of transcription of the self-incompatibility gene. The mitochondrial sequence contains only limited open reading frames and the nuclear sequence has none. There is evidence that additional segments of the mitochondrial clone hybridize to other nuclear sequences. The exchange of sequences between the mitochondrial and nuclear genomes of plants is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00305822
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Recent developments in the molecular genetics and biology of self-incompatibility (1989)
    Springer
    Plant molecular biology 13 (1989), S. 267-271 
    Details
    ISSN: 1573-5028
    Keywords: glycoproteins ; Nicotiana alata ; pollination ; self-incompatibility ; S-locus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A summary of recent work on molecular aspects of self-incompatibility in Nicotiana alata is presented. The amino acid sequences of style proteins corresponding to different S-alleles of N. alata have a high level of homology in some regions and are variable in other regions. The regions of homology include N-terminal sequences as well as most of the glycosylation sites and cysteine residues. The glycosyl substituents may consist of a number of ‘glycoforms’. The isolated style S-glycoproteins inhibit in vitro growth of pollen tubes. The S-glycoproteins tested inhibited the growth of pollen of several S-genotypes, and there was some specificity in the interaction. Heat treatment of the isolated S-glycoproteins dramatically increased their activity as inhibitors of pollen tube growth, although the specificity in the interaction was lost. The nature of the S-allele products in pollen is not yet established.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025314
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    A retrotransposon-like sequence linked to the S-locus of Nicotiana alata is expressed in styles in response to touch (1996)
    Springer
    Molecular genetics and genomics 250 (1996), S. 180-188 
    Details
    ISSN: 1617-4623
    Keywords: Key words gypsy-like retrotransposon ; Nicotiana alata ; S-locus ; Touch-inducible ; Pollination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We have identified a family of repetitive sequences in the genome of Nicotiana alata named Tna1 (Transposon of N. alata). The first element we characterised was on a genomic clone for the N. alata S6-ribonuclease (S6-RNase), a gene required for selfincompatibility in this species. The DNA sequence of this element resembles the integrase domain of retrotransposons of the gypsy class and is most similar to a retrotransposon from Lilium henryi. A transcript present in N. alata styles (self-incompatibility genotype S6S6) hybridized to Tna1 and accumulated in the style following either pollination or touching. This transcript was cloned from a cDNA library and was encoded by a second, partial Tna1 element. Neither the transcribed sequence nor the original Tna1 element contain an open reading frame or is likely to be able to transpose. The second element was mapped using a population of N. alata plants segregating for alleles of the self-incompatibility locus and is closely linked to the S6-allele. The Tna1 element is present in a number of Nicotiana species and appears to have been active at least twice during the evolution of this genus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174177
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...