ISSN:
1432-203X
Schlagwort(e):
Key words Arabidopsis HSP18.2
;
Heat shock protein promoter
;
β-Glucuronidase
;
Nicotiana plumbaginifolia
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract The expression of the Arabidopsis heat shock protein (HSP) 18.2 promoter-β-d-glucuronidase (GUS) chimera gene was investigated in transgenic Nicotiana plumbaginifolia plants during the recovery phase at normal temperatures (20–22 °C) after a heat shock (HS) treatment. GUS activity increased during the recovery phase after HS at 42 °C for 2 h, and maximal GUS activity was observed after 12 h at normal temperatures, at levels 50–100 times higher than the activity immediately after HS. After HS at 44 °C, little GUS activity was observed during the first 20–24 h at normal temperatures, but the activity increased gradually thereafter, to reach a maximum at 40–50 h. After HS at 45 °C, no GUS activity was observed throughout the experimental period. RT-PCR analysis showed that GUS mRNA remained for 10 h after a 2-h HS at 42 °C and for 40 h after a 2-h HS at 44 °C. These findings demonstrate that brief HS treatment, especially at a sublethal temperature, induces a long-term accumulation of HSP-GUS mRNA during the recovery phase.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/s002990050717
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