ISSN:
1432-0983
Keywords:
Botrytis cinerea
;
Hygromycin resistance
;
Non-homologous recombination
;
Phytopathogenic fungus
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A transformation method has been developed for the phytopathogenic fungus Botrytis cinerea. Protoplasts were transformed with pAN7-1 plasmid carrying the Escherichia coli hygromycin phosphotransferase gene (hph), confering hygromycin B resistance, downstream from an Aspergillus nidulans promoter. Molecular analysis showed that transformation resulted in an integration of the plasmid into different regions of the B. cinerea genome and occurred through non-homologous recombination. The frequency was 2–10 transformants per μg of DNA. Transformants expressed phosphotransferase activity confirming that the hph gene conferred the hygromycin-resistance phenotype. All transformants analysed so far proved to be stable after several subcultures without any selective pressure.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00309556
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