ISSN:
1432-2048
Keywords:
Carotenoid biosynthesis, deficiency
;
Chlorophyll photooxidation
;
Chloroplast envelope
;
Mutant (Zea)
;
Norflurazon
;
Zea (carotenoid synthesis)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Plastid-envelope membranes from seedlings ofZea mays L. made carotenoid-deficient by either norflurazon treatment or mutation lack an activity permitting conversion of phytoene to β-carotene. This activity in membrane fractions was measured by coincubation in vitro with a soluble system from spinach chloroplasts capable of converting [14C]isopentenyl pyrophosphate into phytoene. When grown in light, the carotenoid-deficient seedlings lack many soluble chloroplast proteins, including NADP-dependent malic enzyme (EC 1.1.1.40), pyruvate phosphate dikinase (EC 2.7.9.1), and ribulose-1,5-bisphosphate carboxylase (EC 4.1.1.39), but apparently still contain the soluble activities permitting synthesis of phytoene.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01369771
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