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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 117 (1978), S. 265-268 
    ISSN: 1432-072X
    Keywords: Nostoc muscorum ; Blue-green Algal virus ; N-1 ; Lysogeny ; Induction ; Heat ; Mitomycin C
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lysogens were isolated after confluent lysis of the blue-green algaNostoc muscorum by N-1 virus and characterised. The spontaneous liberation of active virus particles in lysogens was not lost after treating them with viricidal concentration of EDTA and virus titre obtained in their cultures was 〉5×103 PFU/ml. The virus adsorbed on the lysogens with a slower rate than on parent alga, although it followed the pattern of a first order reaction. The heat treatment (45°C for 14h) of the lysogens failed to induce lysis, whereas mitomycin C (1–2 μg/ml) was effective in inducing lysis.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 115 (1977), S. 163-167 
    ISSN: 1432-072X
    Keywords: Nostoc virus N-1 ; Temperature ; Adsorption ; One-step growth ; Viral development ; Nostoc muscorum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This study was an attempt to observe the effects of temperature on adsorption and one-step growth of the virus N-1 infecting the nitrogen-fixing cyanobacterium Nostoc muscorum. Adsorption rate was found to be maximum at 40° C whereas no adsorption occurred at 10° C. The Q 10 value was about 2.03 and the energy of activation, Ea was 16.3 kcal/ mole for the adsorption process. The development cycle of the virus was temperature sensitive. With increase in temperature, a gradual increase in inhibition of virus yield i.e. 8.33% at 30° C, 35.3% at 35° C and complete inhibition at 40° C was observed. Out of 7 h latent period, the early 4 h were temperature sensitive and heat treatment had a reversible inhibitory effect on virus development. The temperature treatment did not affect the rise period but burst-size was reduced.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 116 (1978), S. 289-292 
    ISSN: 1432-072X
    Keywords: Blue-green algal virus N-1 ; Adsorption ; Host cell aging ; Killed cells ; Saline magnesium chloride solution ; Amino acids ; pH ; Nostoc muscorum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The adsorption rate of the cyanovirus N-1 infecting the nitrogen-fixing blue-green alga Nostoc muscorum decreased with aging of algal cultures and the virus failed to adsorb to the dead host cells. The adsorption rate declined in saline magnesium chloride solution compared to that in algal growth medium. The addition of amino acids like L-tryptophan and L-phenylalanine failed to enhance the adsorption rate of the virus. Optimal pH of adsorption was 7.6 to 8.1.
    Type of Medium: Electronic Resource
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