ISSN:
1573-5001
Keywords:
Rp-cyclic methylphosphonate
;
Heteronuclear coupling constants
;
Nuclear magnetic resonance spectroscopy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary A method for measuring J(C,P) and J(H,P) coupling constants is presented, based on fitting a target multiplet containing the heteronuclear coupling to a reference multiplet that lacks the heteronuclear coupling. In DNA and RNA oligonucleotides, information on backbone torsion angles can be obtained from these couplings. Experimental multiplets are obtained from 31P-coupled and 31P-decoupled 1H, 13C HSQC spectra of Rp-cyclic methylphosphonate. The accuracy to which the heteronuclear coupling constants can be determined depends on the signal-to-noise ratio of the experimental data and is analyzed in detail.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00176013
Permalink
