ISSN:
1432-0533
Schlagwort(e):
Key words Guillain-Barré syndrome
;
Nuclear bodies
;
Coiled bodies
;
Nuclear size
;
Schwann cells
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Notizen:
Abstract We have examined the reorganization of the cell nucleus in myelin-related Schwann cells (SCs) in a case of acute Guillain-Barré syndrome (GBS). Spinal root samples of the GBS case and human controls were processed for light and electron microscopy. The cytochemical EDTA method for ribonucleoproteins (RNPs) and a specific silver staining technique for nucleolar organizer regions were used on ultrathin sections. In SCs of the GBS case, we observed a significant increase in nuclear size (64.99 ± 10.47 μm2 in the GBS vs 35.07 ± 8.74 μm2 in the controls mean ± SD) accompanying partial decondensation of heterochromatin domains and elaboration of an extensive network of RNP-containing perichromatin fibrils. In addition, the formation of two types of nuclear structures, coiled bodies and nuclear bodies of Bouteille, was induced in SCs of the case of acute GBS. Free coiled bodies were observed in the nucleoplasm and were characteristically stained with both RNP and silver procedures. Typical “simple” and “complex” nuclear bodies were regularly found, sometimes in association with coiled bodies. On the basis of cell nucleus physiology, all of these changes are considered cytological indicators of enhanced transcription and cellular hyperactivity, and they seem to reflect a reactive response of SCs triggered by the constellation of cellular and humoral signals associated with acute GBS.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/s004010050534
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