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  • 1
    Electronic Resource
    Electronic Resource
    A simple and reliable turbidimetric and kinetic assay for alpha-amylase that is readily applied to culture supernatants and cell extracts (1990)
    Springer
    Journal of industrial microbiology and biotechnology 5 (1990), S. 295-301 
    Details
    ISSN: 1476-5535
    Keywords: Alpha-amylase assay ; Turbidity ; Kinetic determination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A simple, reliable and sensitive assay for alpha-amylase activity is reported, together with its theoretical derivation, that overcomes many of the problems encountered with other assays, especially when attempting to assay alpha-amylase activity in crude cell extracts or culture supernatants. The method relies on the reduction in turbidity that occurs upon digestion of a starch suspension with alpha-amylase. The initial rate of decrease in turbidity is shown to be proportional to a wide range of enzyme concentrations, permitting a rapid spectrophotometric and kinetic determination of alpha-amylase activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01578204
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Tandem promoters, tsrp1 and tsrp2, direct transcription of the thiostrepton resistance gene (tsr) of Streptomyces azureus: Transcriptional initiation from tsrp2 occurs after deletion of the — 35 region (1990)
    Springer
    Molecular genetics and genomics 221 (1990), S. 339-346 
    Details
    ISSN: 1617-4623
    Keywords: Streptomyces azureus ; Thiostrepton resistance ; Tandem promoters ; Nuclease S1 mapping ; In vitro transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Nuclease S1 protection experiments indicated that the thiostrepton resistance gene (tsr) of Streptomyces azureus is transcribed from tandem promoters, tsrp1 and tsrp2, that initiate transcription 45 and 173 nucleotides, respectively, upstream of the presumptive translational start codon. The −10 regions of both promoters show similarity to the consensus sequence for the major class of prokaryotic promoters, but the −35 regions do not, although they show some similarity to each other. Replacement of sequences upstream of position −22 relative to the tsrp2 start site with two different DNA segments affected the levels of the tsrp2 transcript but did not alter the tsrp2 initiation site. In vitro transcription assays using RNA polymerase from Streptomyces coelicolor A3(2) also confirmed the location of tsrp2 and identified additional start sites near tsrp2 that were barely detectable with in vivo synthesised RNA. Transcripts corresponding to initiation in vitro at tsrp1 could not be detected, suggesting that additional factors are required for utilisation of this promoter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00259397
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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