ISSN:
0173-0835
Keywords:
Gel retardation
;
Dissociation constant
;
Oligonucleotide association
;
Human HER2
;
Neu oncogene targeting
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Because of the repetitive nature of the sequence, when titrating a G,A-rich, triple helix-forming oligonucleotide (TFO) with increasing concentrations of target duplex in order to obtain the dissociation constant of the complex, a duplex is sometimes first generated at intermediate concentrations of the target. This duplex is based on an imperfect Watson-Crick pairing of the TFO to the pyrimidine-rich strand of the target. An explanation is proposed for this duplex being obtained only in a certain domain of the titration range, before the triple helix becomes predominant.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150191404
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