ISSN:
1612-1112
Schlagwort(e):
Column liquid chromatography
;
Fermentation
;
Microdialysis sampling
;
Monosaccharides and ethanol
;
On-line monitoring
;
Biosensors
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Chemie und Pharmazie
Notizen:
Summary Two amperometric biosensors were used as detection units in liquid chromatography for on-line monitoring of the production of fuel ethanol during a fermentation. Sampling was performed with a microdialysis probe, and the dialysate was introduced into a liquid chromatographic system providing separation of the substrates and the product: three monosaccharides and ethanol. The analytes were detected by two carbon paste electrodes based on pyranose oxidase and alcohol oxidase, respectively, co-immobilised with horseradish peroxidase, operating in parallel at −50 mVvs. Ag/AgCl. The measured linear ranges of the biosensors by direct injection into the LC system were for glucose 0.3–2.5 g L−1, xylose 0.5–6.7 g L−1, galactose 0.5–6.7 g L−1, and ethanol 1.6–11.7 g L−1, respectively. Injection of standard solutions were required to monitor the stability of the biosensors. Due to the strict selectivity of the biosensors, no interference from other compounds in the broth was encountered from other compounds in the broth was encountered in the quantification. The fermentation process was monitored for 16 h. The on-line results were compared with off-line measurements.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF02467486
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