ISSN:
1612-1112
Schlagwort(e):
Column liquid chromatography
;
Optimal mobile phase
;
Gradient elution
;
Microcomputer
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Chemie und Pharmazie
Notizen:
Summary A computer-assisted method is presented for the optimization of separation in gradient elution reversed-phase HPLC. The method is based on a polynomial estimation from nine preliminary experiments according to a two-factor (initial solvent composition C and gradient time T) rectangular design. This is followed by a two-dimension computer scanning technique. Resolution is used as the selection criterion. Good agreement was obtained between predicted data and experimental results.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF02290335
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