ISSN:
1432-072X
Keywords:
Candida
;
Cell Yield
;
Chemostat
;
Growth Efficiency
;
Maintenance
;
Oxygen
;
Respiration
;
Saccharomyces
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Saccharomyces cerevisiae andCandida parapsilosis were grown aerobically and micro-aerobically in continuous culture and the energetic efficiency (Y ATP) and the maintenance energy requirements compared. The bioenergetic parameters were determined using a gas-balance technique, and from cell yield decrements at low dilution rates. The data show that for aerobic cultures, cell yield related to ATP generation (Y ATP) for the two yeasts is similar and close to the value reported originally by Bauchop and Elsden (1960). However, the efficiency of substrate utilization is greater in the case ofC. parapsilosis since the apparent P/O value under aerobic conditions is 1.8 as compared to about 1 forS. cerevisiae. The maintenance energy coefficients based on ATP requirement for aerobic cultures of both yeast were greater than the corresponding values for micro-aerobic cultures; similarly cultures of a petite mutant ofS. cerevisiae had a lower maintenance energy requirement than comparable aerobic wild-type cultures. It is suggested that these differences may reflect the energy requirement for the upkeep of a greater degree of functional complexity in aerobic, wild-type cells, as compared with micro-aerobic or respiratory deficient cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00696220
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