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  • Ob-R  (1)
  • PACS: 24.50.+g Direct reactions – 25.60.Gc Breakup and momentum distributions – 27.20.+n 6 ≤ A ≤ 19  (1)
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  • 1
    ISSN: 1432-0428
    Keywords: Keywords Leptin ; leptin receptor ; Ob-R ; obesity ; sequence variant.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Leptin is an adipocyte-derived blood-borne satiety factor that acts on its cognate leptin receptor (Ob-R) in the hypothalamus, thereby regulating food intake and energy expenditure. To explore whether mutations in the Ob-R gene cause obesity in humans, we have searched for mutations in the gene for Ob-Rb, a biologically active receptor isoform, in obese Japanese subjects. We have also examined associations between such mutants and obesity in the Japanese. Genomic DNAs were used as templates in polymerase chain reaction (PCR) with primers selected to amplify exons 2 to 20 of the human Ob-Rb gene. Direct sequence analysis of the PCR products revealed 7 nucleotide sequence variants (Lys109Arg, Gln223Arg, Ser343Ser, Ser492Thr, Lys656Asn, Ala976Asp, and Pro1019Pro) in the Ob-Rb coding region from 17 obese Japanese subjects with a family history of obesity (BMI 39.3 ± 8.4 kg/m2). No missense and nonsense mutations were found such as those in Zucker fatty (fa/fa) rats and Koletsky (fa k /fa k) rats. Nucleotide substitutions occurred at relatively high frequencies at codons 109, 223, 976, and 1019 (79, 91, 100, and 85 %, respectively). Allele frequency of each variant determined by PCR-RFLP and PCR-single strand conformation polymorphism analyses showed no significant differences between 47 obese (BMI 35.1 ± 6.5 kg/m2) and 68 non-obese (BMI 21.6 ± 2.2 kg/m2) subjects. The present study represents the first report of sequence variants of the Ob-Rb gene in the Japanese and provides evidence against either obesity-causing mutations or association of sequence variants with obesity in obese Japanese subjects. [Diabetologia (1997) 40: 1204–1210]
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1434-601X
    Keywords: PACS: 24.50.+g Direct reactions – 25.60.Gc Breakup and momentum distributions – 27.20.+n 6 ≤ A ≤ 19
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract: Elastic scattering and inclusive breakup of 6Li particles on 12C, 58Ni, 90Zr, and 208Pb targets are measured at 100A MeV. The elastic scattering data are compared with single channel and Coupled Discretized Continuum Channels calculations. The coupling-effect between the elastic and the breakup channels is important even at an incident energy of 100A MeV and cannot be neglected. The inclusive breakup data are investigated for orbital dispersion effects which are found to be less significant at 100A MeV. The longitudinal momentum distributions are broader than predicted by theoretical expectations.
    Type of Medium: Electronic Resource
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