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  • Antisense phosphodiester oligonucleotide  (1)
  • PACS. 34.50.-s Scattering of atoms, molecules, and ions - 36.40.-c Atomic and molecular clusters - 64.60.Qb Nucleation  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Biodistribution of iodine-125 tyramine transforming growth factor α antisense oligonucleotide in athymic mice with a human mammary tumour xenograft following intratumoral injection (1996)
    Springer
    European journal of nuclear medicine 23 (1996), S. 448-452 
    Details
    ISSN: 1619-7089
    Keywords: Transforming growth factor α ; Antisense phosphodiester oligonucleotide ; Radioiodine labelling ; Biodistribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The Watson-Crick base pairing rule provides the underlying principle for the antisense (AS) approach to inhibiting gene expression. Transforming growth factor α (TGFα) was the first growth factor to be associated with tumorigenesis, thus making the TGFα (mRNA) a potential target for AS therapy and offering the potential for monitoring of the progression of malignancy by non-invasive imaging with radiolabelled AS phosphodiester. Probe labelling and biodistribution were studied in the present report. A 23-mer oligonucleotide sequence was synthesized and grafted in 5′ with a tyramine group which was further radioiodinated. The radiolabelled AS was injected intratumorally in mammary tumour-bearing BALB/c mice (3 weeks after inoculation of 7·106 NS2T2A mammary cells). Biodistribution was monitored by sequential scintigraphy and organ radioactivity after autopsy. The 5′ tyramine group allowed specific and stable radiolabelling of the AS with125I. The125I AS oligonucleotide was rapidly cleared from the tumour by intestine and kidneys. Four hours after intratumoral injection, 6.5%±1.5% of the dose was retained in the tumour as non-degraded125I AS. It is concluded that 5′ tyraminylated AS provides information on the biodistribution of AS oligonucleotide following intratumoral injection. These data will contribute to the pharmacology of AS oligonucleotides which can be used for therapy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01247375
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  • 2
    Electronic Resource
    Electronic Resource
    Evidence for dependence of the sticking cross-section of atoms on small and medium-size van der Waals clusters (2000)
    Springer
    The European physical journal 8 (2000), S. 265-272 
    Details
    ISSN: 1434-6079
    Keywords: PACS. 34.50.-s Scattering of atoms, molecules, and ions - 36.40.-c Atomic and molecular clusters - 64.60.Qb Nucleation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract: This paper introduces a simple model describing the cluster growth in supersonic expansions. The predicted terminal mean cluster size is compared to the available data in the case of argon. The agreement between the model and the experimental results requires that the cross-section describing the sticking of an atom on a cluster of size N scales like with in the range 0.34-0.44, well below the predicted by the simplest geometrical scaling argument. We explain this unexpected result in two steps. First, using Monte Carlo simulations, we check that the potential between an atom and a cluster is accurately represented by the Gspann and Vollmar potential, even at finite temperature. Then, using Langevin's approximation, we show that the sticking cross-section scales like N 1/3 for small to moderate N values and switches to the geometric scaling N 2/3 for very large N values. The crossover between these two scalings occurs when for argon, but the mean exponent over the size range 1-104 is 0.46. This N scaling of the sticking cross-section should play an important role whenever condensation is important as it modifies the kinetics of the early stages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100530050035
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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