ISSN:
1432-072X
Keywords:
Pseudomonas aeruginosa
;
PAO1
;
Phage PIK
;
Receptor
;
Lipopolysacharide
;
Lipopolysaccharide inactivation by sugars
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Receptor for phage PIK specific for Pseudomonas aeruginosa strain PAO1 was studied. Phage PIK was strongly inactivated by lipopolysaccharide (LPS)_in vitro, exhibiting a PhI50 of 4.8 μg/ml. Further it was noted that this inactivation by LPS was reduced to 50% by several mono- and disaccharides when tested in vitro. d-glucosamine, d-mannose and l-rhamnose were found to be most effective at the concentration of 0.045 M, 0.25 M and 0.35 M respectively. This suggests the possibility that phage PIK receptor in LPS contains d-mannose, l-rhamnose and d-glucosamine. Either one of the former two could be located at a terminal position alpha-linked to the adjacent residue or located internally in the polysaccharide chain linked through its C-4 position. A theoretical approach to the interpretation of phage cell interaction was also investigated.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00408026
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