ISSN:
1572-994X
Keywords:
PVY
;
capsid protein
;
nucleotide sequence
;
potyvirus
;
cloning
;
restriction endonuclease
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The nucleotide sequence of the 3′ terminal region of potato virus Y (PVY) was determined. Starting with a poly(A) tail of 18 residues a non-coding region of 335 nucleotides precedes the region encoding for the virus coat protein (cp) 801 nucleotides long ending with a TGA. This region was located by comparing the predicted amino acid sequence with the one determined for the PVY capsid protein by Shukla et al. (1). Both sequences contained 267 amino acids sharing about 94% homology. They differ, however, at several positions presumably due to base transitions within their respective nucleotide sequences. Restriction endonuclease sites in and around the cp coding region were identified.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00572704
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