ISSN:
1432-203X
Keywords:
Key wordsLycopersicon esculentum
;
Salt stress
;
Peroxidase
;
Cell suspension
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The expression pattern of a peroxidase – (EC 1.11.1.7) encoding gene, TPX1, was studied in NaCl-adapted and unadapted tomato (Lycopersicon esculentum) cells growing in suspension culture. We followed the transcript level during the cell growth cycle and under different salt treatments. There was a cell-cycle-dependent TPX1 expression, the maximum level of transcripts coinciding with the period of cell enlargement in both salt-adapted and unadapted tomato cells. However, enzyme activity of the encoded isoperoxidase was only detected in the culture medium of the salt-adapted cells. NaCl treatment decreased TPX1 transcripts in both cell lines, but the threshold concentration for this down-regulation was higher in adapted cells. In conclusion, salt-adapted tomato cells show significant changes in comparison with unadapted cells in the expression of a gene encoding a cell-wall-targeted peroxidase.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050642
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