ISSN:
1573-4978
Keywords:
chromatin structure
;
chromosomal proteins
;
DNA-binding proteins
;
nuclease sensitivity
;
Physarum polycephalum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have identified two novel chromosomal proteins from Physarum polycephalum using a protein blotting DNA-binding assay. A fraction of these proteins was readily released from nuclei by solutions of moderate ionic strength (0.15 N–0.35 M NaCl) or mild nuclease treatment and appear associated with chromatin that is nucleosome-free. A significant proportion of these proteins, however, was not released from nuclei by solutions of high ionic strength (1.6 M NaCl) or treatment with excess nuclease. These results suggest that these chromosomal proteins are distributed between transcriptionally-competent and inert domains of chromatin. Both proteins preferentially and tenaciously bound duplex DNA, especially to the alternating B-DNA conformation displayed by the synthetic sequence, poly d(A-T).poly d(A-T).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00419756
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