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  • Physics  (2)
  • Polymer and Materials Science  (2)
  • Arginine  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Factors that influence Agrobacterium rhizogenes-mediated transformation of broccoli (Brassica oleracea L. var. italica) (2000)
    Springer
    Plant cell reports 19 (2000), S. 994-999 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Key words Agrobacterium rhizogenes ; Brassica oleracea ; Transformation ; Arginine ; Acetosyringone
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  An improved broccoli transformation system was developed by optimising several factors that affect the rate of effective Agrobacterium-mediated transformation. Leaf explants of cultivar Shogun were co-cultivated with Agrobacterium rhizogenes strain A4T harbouring the binary vector pART278. The T-DNA of this binary vector contains a neomycin phosphotransferase II (NOS-NPTII-NOS) gene for kanamycin resistance and a β-glucuronidase (35S-GUS-OCS) gene. Several media and factors were evaluated including combinations of arginine, mannopine, acetosyringone and the use of feeder cell layers. The new protocol includes the use of 200 μm acetosyringone in LB medium for bacterial growth, the use of a Brassica campestris feeder cell layer, 10 mm mannopine and 50 μm acetosyringone in the co-cultivation medium and 1 mm arginine in the selection medium. The use of this optimised protocol produced transformation rates of 33% in preliminary experiments transforming broccoli with the antisense 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase gene from pTOM13.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002990000221
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  • 2
    Digitale Medien
    Digitale Medien
    D2-m-carborane siloxanes. V. Structure-property relationships (1977)
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Physics Edition 15 (1977), S. 723-732 
    Details
    ISSN: 0098-1273
    Schlagwort(e): Physics ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Physik
    Notizen: D2-m-decacarborane dimethylsiloxane has unusual thermal stability and resistance towards reversion, however, this material exhibits a crystalline phase, Tm at 68°C. To obtain an elastomeric material, this crystallinity is disrupted by replacing 30 to 50% of the m-carborane with p-carborane moieties or by the incorporation of phenyl moieties on the polymer backbone. The latter approach is preferred since improved thermo-oxidative stability accompanies this modification. Correlation of the glass transition temperature and thermo-oxidative stability with the polymer structure are presented.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/pol.1977.180150412
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  • 3
    Digitale Medien
    Digitale Medien
    D2-meta-carborane-siloxanes. IV. Synthesis of linear, high molecular weight polymers (1977)
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Chemistry Edition 15 (1977), S. 2229-2238 
    Details
    ISSN: 0360-6376
    Schlagwort(e): Physics ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: A new facile route to linear, high molecular weight D2-meta-decacarborane-siloxanes was developed based on the condensation reaction between bisureidosilanes and carborane disilanol. By using NMR to monitor the reaction stoichiometry, polymers with molecular weight ≥250,000 have been prepared. Moreover, because of the mild reaction conditions, structural modifications of the polymer backbone have been readily achieved. These linear, high molecular weight polymers overcome the fabrication difficulties encountered in previously prepared D2-polymers.
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/pol.1977.170150914
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