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  • 1
    Electronic Resource
    Electronic Resource
    Isolation and identification of a new, naturally occurring cytokinin (6-benzylaminopurineriboside) from an anise cell culture (Pimpinella anisum L.) (1983)
    Springer
    Planta 159 (1983), S. 222-225 
    Details
    ISSN: 1432-2048
    Keywords: Cell culture (cytokinin content) ; Cytokinin (6-benzylaminopurineriboside) ; Pimpinella
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new, naturally occurring cytokinin was isolated from a cell culture of anise (Pimpinella anisum L.). Its structure was determined as 6-benzylaminopurine riboside by the use of gas chromatography and mass spectrometry. Quantitation of this new plant hormone was accomplished by single-ion-monitoring measurements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397528
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation and quantitation of isopentenyladenosine in an anise cell culture by single-ion monitoring, radioimmunoassay and bioassay (1983)
    Springer
    Planta 159 (1983), S. 216-221 
    Details
    ISSN: 1432-2048
    Keywords: Cell culture (cytokinin content) ; Cytokinin isolation and quantitation ; Pimpinella
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using three different techniques, isopentenyladenosine was identified and quantitated in an anise cell line (Pimpinella anisum L.), growing on a medium without cytokinin. A method to quantitate cytokinins was developed which utilizes extraction of cytokinins in the presence of a deuterated reference compound and subsequent quantitation of the cytokinins by single-ion monitoring on a gas chromatography-mass spectrometry system. The results were compared with those obtained by radioimmunoassay as well as a bioassay. Quantitations by gas chromatography-mass spectrometry and radioimmunoassay correlated well, whereas the estimates by the bioassay gave considerably lower values.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397527
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Endogenous cytokinins during embryogenesis in an anise cell culture (Pimpinella anisum L.) (1984)
    Springer
    Planta 161 (1984), S. 240-245 
    Details
    ISSN: 1432-2048
    Keywords: Cell culture ; Cytokinin and embryogenesis ; Embryonic/proembryonic development ; Pimpinella
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Endogenous levels of cytokinins in an anise cell culture were determined by the use of radioimmunoassay and gas chromatography-mass spectrometry in combination with single-ion monitoring, during proembryonal and embryonal development. In both cultures the highest cytokinin levels were correlated with logarithmic growth (embryonal: isopentenyladenosine, 4 ng g-1 fresh weight [FW]; isopentenyladenine, 1.4 ng g-1 FW; zeatin, 3.6 ng g-1 FW; proembryonal: isopentenyladenosine, 58.3 ng g-1 FW; isopentenyladenine, 7.9 ng g-1 FW; zeatin 11.1 ng g-1 FW). The proembryonic culture medium but not the embryonic culture medium contained isopentenyladenosine up to 28 pg ml-1 during logarithmic growth. No correlation between different embryonic stages and the endogenous cytokinin level was obvious.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00982919
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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