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Polycytidylate and poly(7-deazaguanylate): A pair of complementary templates (1994)

Rembold, Hansjörg ; Robins, Roland K. ; Seela, Frank ; [et al.]

Springer

Journal of molecular evolution 38 (1994), S. 211-214

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ISSN: 1432-1432

Keywords: Template-directed synthesis ; Polycytidylic acid ; Poly(7-deazaguanylic acid) ; Oligocytidylates ; Chemical evolution

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract We have studied the template-directed oligomerization on polycytidylic acid of the 5′-phosphoro(2-methyl)imidazolides of a number of analogues of guanosine. None of the analogues reacted as efficiently as the original guanosine compound, and only the 7-deazaguanosine analogue gives a detectable yield of oligomers. Similar results are described for a reaction involving the intramolecular template-directed elongation of a short oligocytidylate primer. Oligocytidylates containing five or more cytidylate residues are extended on the single-stranded regions of poly(G). In the present study we show that these oligocytidylates are extended efficiently by reaction with cytidine-5′-phosphoro(2-methyl) imidazolide on a poly(7-deazaguanylic acid) template. The products are considerably longer than those obtained using a polyguanylic acid template. We believe that the formation of a tetrahelix inhibits the latter reaction, while poly(7-deazaguanylate) does not aggregate and, therefore, acts as a more efficient template. This work identifies for the first time a pair of homopolymers each of which facilitates the template-directed elongation of the other.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00176083

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Field ionization mass spectrometry - I. NucleosidesThis paper is also Part IX in the series ‘Structural Biochemistry’. For Part VIII see g. R. Pettit and S. K. Gupta, J. Chem. Soc. (C), 1208 (1968). (1969)

Brown, Peter ; Pettit, George R. ; Robins, Roland K.

Chichester : Wiley-Blackwell

Biological Mass Spectrometry 2 (1969), S. 521-531

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ISSN: 0030-493X

Keywords: Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: The field ionization mass spectra of a series of nucleosides are reported, and compared with spectra obtained by conventional electron-impact ionization. The latter are complex, with structurally significant molecular ion and sugar cleavage peaks often of low intensity or completely absent. In contrast, the field ion spectra are extremely simple, with all except guanosine (highest mass peak [M - 18]) showing intense molecular ion peaks, and the characteristic sugar (S) and base (B + H) cleavage products as the only other important fragments.

Additional Material: 10 Ill.