ISSN:
1040-0397
Schlagwort(e):
Redox mediators
;
Immunoglobulin G
;
Polyvinyl ferrocene
;
Butyl ferrocene
;
lmmunosensor model
;
Chemistry
;
Polymer and Materials Science
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Chemie und Pharmazie
Notizen:
As a step towards a voltammetric immunoassay with antibodies directly attached onto the electrode surface, the mediated oxidation of a redox active label has been investigated with a model system. Butyl ferrocene served as a model for the mediator and was coimmobilized onto a glassy carbon electrode within a layer of bovine immunoglobulin G, which mimiked the antibody. An overlaid polyvinyl ferrocene layer was employed to simulate a ferrocene-tagged high molecular weight antigen bound to the immobilized antibodies. A mediated electron transfer could only be demonstrated with butyl ferrocene as mediator, whereas other compounds failed to support an electron transfer between the polyvinyl ferrocene layer and the electrode through the layer of immunoglobulin.
Zusätzliches Material:
6 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/elan.1140080209
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