ISSN:
1573-4994
Keywords:
Polystyrene–polyethyleneglycol microbeads
;
translational mobility
;
dynamic excimers
;
pyrenebutyric acid
Source:
Springer Online Journal Archives 1860-2000
Topics:
Physics
Notes:
Abstract Polyethyleneglycol (PEG) chain mobility in gelatineous microbeads is investigated by means of dynamic excimer formation. Pyrenebutyric acid (PYB) is covalently linked to the chain ends as probe molecule. Excimer formation is monitored by steady-state and time-resolved fluorescence spectroscopy in the presence of a series of liquid phases and in the dry state. PYB concentration in the beads is varied over three orders of magnitude up to c = 6·10−2 M. The concentration is derived from absorption measurements in stirred bead suspensions, considering the deviations from Lambert–Beer's law in heterogeneous systems. Excimer formation is found to be a dynamic process in the presence of liquid phases which solvate both the polymer and the fluorophore. The collisional rate constant, k DM, is of the order of k DM-values of PYB in homogeneous solutions, indicating a high translational mobility. Excimer-to-monomer intensity ratios are in general accordance with the solvation capacity of the liquid phase. In the dry state excimer formation is found only at high PYB concentrations, c ≈ 3·10−2 M. It is concluded that this excimer emission arises from aggregated PYB, since corresponding fluorescence response curves show no rise time.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1022506627591
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