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Somaclonal variation in Solanum tuberosum detected at the molecular level (1985)

Landsmann, J. ; Uhrig, H.

Springer

Theoretical and applied genetics 71 (1985), S. 500-505

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ISSN: 1432-2242

Keywords: Somaclonal variation ; Potato ; Tissue-culture ; Ribosomal RNA genes ; Repetitive DNA

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary The many reports of phenotypic variation among plants regenerated from tissue culture suggest underlying alterations at the DNA level. This hypothesis was tested with protoplast-derived Solanum tuberosum plants. Random potato-DNA clones were used to probe the genome of individual plants at specific sites. Two out of twelve plants were shown to be variant by Southern-hybridisation with one of the tester-clones. As this clone turned out to represent 25S-rDNA, both somaclonal variants can be regarded as mutants deficient in ribosomal RNA-genes.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00251196

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