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  • Proteases  (1)
  • calcium  (1)
Materialart
Erscheinungszeitraum
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 123 (1979), S. 23-35 
    ISSN: 1432-072X
    Schlagwort(e): Yeast ; Compartmentation ; Vacuoles ; Lysosome ; Cytosol ; ATPase ; Phosphatases ; Proteases ; Polyphosphate
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Almost all the vacuoles (about 95%) remained intact after “polybase-induced lysis” of the yeast protoplasts. These vacuoles could be sedimentated together with other cell organelles which were equally well preserved, leaving as a supernatant a cytosol fraction which was essentially uncontaminated by the contents of disrupted vacuoles. After density gradient centrifugation more than half of the vacuoles were recovered in a fraction which was highly purified as judged from the measurement of several marker enzymes and from light and electron microscopic observations. Polyphosphate, which has been shown to be located exclusively in the vacuolar sap of protoplasts, was used as a vacuolar marker to determine the yields of vacuoles in the different fractions obtained from the density gradients. It was also used to assess the overall distribution of lytic enzymes in the cytosol and in the vacuome. The results indicate that the following enzyme activities are mostly, if not exclusively (〉90%), located in the vacuome, probably all in the typical large vacuoles present in the protoplasts: exo-and endopolyphosphatase, proteases A and B, carboxypeptidase Y, an aminopeptidase, RNase, α-mannosidase, and phosphatases which hydrolyze a number of different substrates. The polyphosphatases are thus in the same compartment as the polyphosphate. The activities of some other hydrolases, notably of a Mg2+ dependent, Oligomycin and NaN3 insensitive ATPase and alkaline phosphatase, were partially associated with the vacuoles. The activities of pyrophosphatase, tripolyphosphatase, α-glucosidase, and aminopeptidase active in the presence of EDTA, were located almost exclusively in the soluble, cytosolic fraction.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    ISSN: 1573-0867
    Schlagwort(e): calcium ; compost ; irradiation ; magnesium ; manure ; sewage sludge
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Application of organic waste influences crop uptake of Ca and Mg and soil extractability, depending on the nature of the crop and the waste. Four organic wastes: (i) digested sewage sludge (DSS), (ii) irradiated sludge (DISS), (iii) composted sludge (DICSS), and (iv) composted livestock manure (CLM) were applied for two years at rates of 10, 20, 30, and 40 Mg solid ha-1 year-1. Fertilizers N and K were applied to the control treatment (CT), as well as to the waste treatments to supplement crop growth across all treatments, so that these nutrients were not treatment variables. Calcium and Mg concentrations in the tissue of lettuce, bean pods and petunias in 1990 and two cuts of lettuce in 1991, and the CH3COONH4-extractable soil Ca and Mg were determined. Concentration of Ca and Mg in bean pods did not change to the waste application. Calcium concentration in bean pods was less than half of that in other crops. Magnesium concentration in bean pods and petunias was same, but was much lower than in lettuce. Application of DSS, in general, increased Ca concentration in the crops more than did other wastes. The extractable soil Ca was positively correlated with Ca applied with DISS (r=0.453, P〈0.05). Although only a limited amount of Ca was supplied with CLM at the rate of 10 Mg solid ha-1(40 kg Ca ha-1), Ca concentration in petunias increased significantly, then, decreased with increased Ca application (r=0.453, P〈0.05). A similar pattern with CLM was found in the extractable soil Ca. The waste application from all the sources had no influence on crop Mg concentration in 1990, possibly due to low Mg concentration in the wastes. While continuously applied DSS and DISS in 1991 linearly increased Mg concentration in both cuts of lettuce (r=0.867, P〈0.01; r=0.670, P〈0.01 and r=0.671, P〈0.01; r=0.665, P〈0.01 for first cut and second cut of lettuce with DSS and DISS application respectively), application of CLM decreased Mg concentration in first cut lettuce. The patterns of extractable soil Mg were opposite to crop Mg concentration, as the extractable soil Mg linearly increased with CLM, and decreased with the high rate of DSS application. The ability of wastes to supply N was an important factor influencing crop Σ cations (K, Ca and Mg) uptake.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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