ISSN:
1423-0127
Keywords:
γ-Aminobutyric acid
;
L-glutamate decarboxylase
;
Protein phosphorylation
;
Neurotransmitter synthesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract In the brain, the γ-aminobutyric acid (GABA) level is primarily controlled by the activity of its synthesizing enzyme,L-glutamate decarboxylase (GAD). At present, mechanisms responsible for regulation of GAD activity remain largely unknown. Here we report that GAD activity is inhibited by conditions favoring protein phosphorylation, and this inhibition can be reversed by phosphatase treatment. Furthermore, this inhibition appears to result from the suppression of a Ca2+-dependent phosphatase. Phosphorylation of GAD is demonstrated by direct incorporation of32P into the GAD protein. These results suggest that GAD activity in the brain is inhibited by phosphorylation and activated by dephosphorylation. A model for regulation of GABA synthesis related to neuronal excitation is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02253308
Permalink
