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  • 1
    Electronic Resource
    Electronic Resource
    RFLP analysis of asymmetric somatic hybrids between Solanum tuberosum and irradiated S. brevidens (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 754-760 
    Details
    ISSN: 1432-2242
    Keywords: Solanum brevidens ; Asymmetric somatic hybrids ; Protoplast fusion ; Restriction fragment length polymorphism ; Potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nuclear genome composition of five asymmetric somatic hybrids, obtained by fusion of leaf protoplasts from Solanum tuberosum and gamma-irradiated leaf protoplasts from S. brevidens, have been analyzed at the molecular level. An analysis of 21 loci using linkage group-specific restriction fragment length polymorphism (RFLP) was included in the study. All five hybrids contained a complete set of the loci studied from S. tuberosum. The degree of elimination of alleles from the irradiated S. brevidens donor genome ranged from 10–65% in the five asymmetric hybrids analyzed. The detection of incomplete chromosomes, as well as non-parental bands in Southern hybridizations with RFLP markers, revealed extensive chromosome rearrangements in the asymmetric hybrids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222666
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Production of asymmetric hybrids between Solanum tuberosum and irradiated S. brevidens (1993)
    Springer
    Theoretical and applied genetics 85 (1993), S. 729-734 
    Details
    ISSN: 1432-2242
    Keywords: Protoplast fusion ; Gamma irradiation ; Partial genome transfer ; Solanum tuberosum ; Solanum brevidens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Asymmetric somatic hybrids were obtained by fusion of Solanum tuberosum (PDH40) protoplasts with 300- or 500-Gy irradiated protoplasts of S. brevidens. These radiation doses were sufficient to prevent the growth of the S. brevidens protoplasts. Putative hybrids were selected on the basis of phenotype from regenerated shoots and identified with a S. brevidens-specific probe. From these, 31 asymmetric hybrids were confirmed by morphological characteristics, isoenzyme patterns and RFLP analysis. The morphology of the asymmetric hybrids was intermediate between that of S. tuberosum and symmetric hybrids of both species (obtained without irradiation treatment). Chromosome counts from 17 asymmetric hybrids showed that the chromosome number of the hybrids ranged from 31 to 64. The asymmetric hybrids probably had one or two genome complements (i.e. either 24 or 48 chromosomes) from S. tuberosum and 7–22 chromosomes from S. brevidens. There was no clear correlation between the radiation dose and the degree of elimination of the S. brevidens genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225012
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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