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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Planta 135 (1977), S. 199-201 
    ISSN: 1432-2048
    Keywords: Cell suspension culture ; Fusicoccin ; 3-O-Methylglucose uptake ; Potassium ion uptake ; Proton extrusion ; Protoplasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have assayed the capacity of the fungal toxin fusicoccin to induce some of its characteristic effects (acidification of the medium, stimulation of K+, and of 3-O-methyl-D-glucose uptake) in cell suspensions of Parthenocissus tricuspidata (Siebold et Zucc.) Planchon, Acer pseudoplatanus L. and Oryza sativa L., and in protoplast suspensions prepared from leaves of Nicotiana tabacum L. and Spinacia oleracea L. or from cultures of P. tricuspidata. Evidence is presented showing that all tested biological materials respond to the addition of fusicoccin. The observation that the toxin is also active on protoplasts indicates that the cell wall is not involved in the mechanism of action of fusicoccin.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 78 (1989), S. 113-118 
    ISSN: 1432-2242
    Keywords: Nicotiana plumbaginifolia ; Protoplasts ; Gene transfer ; Repeated DNA sequences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Highly repeated nuclear DNA sequences from leaves of Nicotiana plumbaginifolia were cloned in pBR322 and tested for their effect on direct gene transfer in protoplasts of the same organism. Protoplasts were prepared from suspension cultures and were incubated in the presence of the plasmid pHP23 carrying the kanamycin resistance gene APH(3′)II and in the presence of the plasmids carrying the cloned sequence. DNA uptake was induced by a polyethyleneglycol (PEG) treatment. Out of the 22 tested clones, 3 significantly stimulated the frequency of appearance of transformed colonies. DNA was extracted from some of the kanamycin-resistant calli obtained by co-transformations. Dot-blots have shown that the stimulatory effect on transformation frequency is often accompanied by a consistent increase in integrated genes sequences.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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