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  • 1975-1979  (3)
  • Biochemistry and Biotechnology  (2)
  • Proximal tubule  (1)
  • Gene expression
  • 1
    Digitale Medien
    Digitale Medien
    Microbodies of the rat renal proximal tubule: Ultrastructural and cytochemical investigations (1975)
    Springer
    Cell & tissue research 157 (1975), S. 283-305 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Microbodies ; Kidney ; Proximal tubule ; Ultrastructure ; Cytochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The present study was undertaken to provide a detailed morphological and cytochemical characterization of the microbodies of the rat renal proximal tubule following perfusion fixation. The following observations were made: 1) Two basic types of microbodies (Mb-I and Mb-II) can be identified. Mb-I have both circular and tubular profiles which are located peripherally within the granular matrix of these microbodies. Mb-II have marginal plates and crystalloid inclusion in addition to circular and tubular profiles. 2) Circular and tubular profiles, 100 nm in diameter, described by previous investigators as being infrequent in occurrence, are the most consistent morphological characteristic of rat renal microbodies after perfusion fixation. These profiles have a homogeneous center surrounded by a double or single ring of granules. The uniform size and spacing of these granules within profiles establish a basic 100 Å periodicity found in both types of microbodies. 3) Evidence is presented which suggests that both “nucleoids” and “tubular protrusion rods” as described by other investigators of the rat renal microbodies may result from poor fixation and/or osmotic stress. 4) The density of the matrix of Mb-I is, in contrast to previous reports, greater than the density of adjacent mitochondria. 5) Marginal plates or crystalloid inclusions were demonstrated in some microbodies (Mb-II) of all the rats studied; periodicities of 100, 200, and 300 Å were identified within these structures. 6) Both types of microbodies were positive for catalase activity, but were negative for acid phosphatase activity. On the basis of both morphological and cytochemical criteria, it seems plausible that these two populations of renal microbodies (Mb-I and Mb-II) represent a morphological and functional continuum.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00225521
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  • 2
    Digitale Medien
    Digitale Medien
    Efficient biokinetic experimental designs (1975)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 17 (1975), S. 557-570 
    Details
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Of the experimental methods available for obtaining data to estimate the biological kinetic parameters μm, Ks, and Yeach requires considerable experimental effort, yet often yields somewhat imprecise estimates of the parameters, particularly Ks. Therefore it would be worthwhile to seek ways to get parameter estimates of greater precision using less experimental effort. The precision of parameter estimates is strongly dependent, upon the settings of the independent, variables used in the experiments. This dependence is explained and an attempt made to show how experimental settings can be determined that lead efficiently to precise parameter estimates with minimal effort.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bit.260170408
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  • 3
    Digitale Medien
    Digitale Medien
    Using multiresponse data to estimate biokinetic parameters (1975)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 17 (1975), S. 571-583 
    Details
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Biokinetic parameters are usually calculated from slopes and intercepts taken from plots of experimental data. One response at an item is plotted and used for parameter estimation. Aside from problems that may be caused by transformations made when the data are plotted, this approach has the weakness of not using all the data simultaneously when there is more than one response. This paper shows how multiresponse biological data can be handled to get parameter estimates that are much more precise than those obtained using conventional methods.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bit.260170409
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