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  • 1
    Electronic Resource
    Electronic Resource
    Style antigens of Prunus avium L. (1981)
    Springer
    Planta 153 (1981), S. 125-129 
    Details
    ISSN: 1432-2048
    Keywords: Antigen (style) ; Prunus ; Self-incompatibility ; Style antigens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Antiserum to a protein fraction of an extract of mature styles of P. avium cv. Lambert (S 3 S 4) was raised in rabbits. Two major antigenic components of the style extracts were detected by immunoelectrophoresis and immunodiffusion. The presence of one antigen (S-antigen) correlated with a particular S-genotype (S 3 S 4). This antigen is restricted to mature styles of P. avium. The second antigen (P-antigen) was detected in styles of all Prunus species examined, but not in styles of other species of the Rosaceae. The S-antigen is positively charged and the P-antigen negatively charged at pH 8.8.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384093
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Tissue-specific antigens secreted by suspension-cultured callus cells of Prunus avium L. (1981)
    Springer
    Planta 153 (1981), S. 115-124 
    Details
    ISSN: 1432-2048
    Keywords: Antigen ; Callus ; Cell suspension culture ; Prunus ; Rosaceae ; Secretions, extracellular ; Self-incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A number of antigenic components are secreted into the medium by P. avium callus cells derived from different tissues and grown in suspension culture. These antigens have been detected using antiserum raised in rabbits to a protein fraction secreted by P. avium leaf callus. One antigen is specific to leaf tissue and is secreted by callus cells derived from stem, pistil and anthers as well as leaves. A second antigen is, in intact organs, restricted to styles of a particular self-incompatibility (S) genotype, but is also secreted by callus cells derived from the leaf. Another antigen, apparently not organ-specific, is secreted by all calli tested, including Rosa (cv. Paul's Scarlet).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384092
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Isolation and partial characterization of components of Prunus avium L. styles, including an antigenic glycoprotein associated with a self-incompatibility genotype (1982)
    Springer
    Planta 156 (1982), S. 505-516 
    Details
    ISSN: 1432-2048
    Keywords: Antigen ; Glycoprotein ; Pollination ; Prunus ; S-allele ; Self-incompatibility ; Style mucilage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several components of buffer extracts of Prunus avium L. styles (cv. Lambert, S 3 S 4) have been isolated and partially characterized: the major component is a glycoprotein (molecular weight approx. 90,000; 95% protein, 5.4% carbohydrate). A “sticky” uronic-acid-containing component and an arabinogalactan are also present. Two minor components are an antigenic glycoprotein associated with the self-incompatibility genotype (Antigen S) and a component found in styles of all Prunus species (Antigen P). The isolated glycoproteins have a substantial carbohydrate content (Antigen P 17.2%; Antigen S 16.3%), and have apparent molecular weights of 32,000 (Antigen P) and 37,000–39,000 (Antigen S). They are antigenically quite distinct. Material corresponding to Antigen S is secreted into the medium of suspension-cultured callus cells raised from both leaf and stem of P. avium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392772
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    The effect of isolated components of Prunus avium L. styles on in vitro growth of pollen tubes (1982)
    Springer
    Planta 156 (1982), S. 517-519 
    Details
    ISSN: 1432-2048
    Keywords: Pollen tube growth (in vitro), inhibition ; Prunus ; S-allele ; Self-incompatibility ; Style components
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A number of components isolated from styles of P. avium cv. Napoleon (S 3 S 4) have been tested for their capacity to influence in vitro growth of pollen tubes from fresh and stored pollen (cv. Napoleon (S 3 S 4)). An antigenic glycoprotein (Antigen S) is a potent inhibitor of in-vitro pollen tube growth, causing a 65% reduction in tube length at a concentration of 20 μg/ml. None of the other style components were effective inhibitors of pollen tube growth; neither were proteins of animal origin such as histone, serum albumin, cytochrome C, and the glycoproteins ovalbumin and thyroglobulin, effective inhibitors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392773
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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