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  • 1
    Electronic Resource
    Electronic Resource
    A method to measure genetic distance between allogamous populations of alfalfa (Medicago sativa) using RAPD molecular markers (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 406-412 
    Details
    ISSN: 1432-2242
    Keywords: Key words Medicago sativa ; RAPD ; Cultivars ; Genetic distance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Alfalfa (Medicago sativa L.) is a forage legume of world-wide importance whose both allogamous and autotetraploid nature maximizes the genetic diversity within natural and cultivated populations. This genetic diversity makes difficult the discrimination between two related populations. We analyzed this genetic diversity by screening DNA from individual plants of eight cultivated and natural populations of M. sativa and M.  falcata using the RAPD method. A high level of genetic variation was found within and between populations. Using five primers, 64 intense bands were scored as present or absent across all populations. Most of the loci were revealed to be highly polymorphic whereas very few population-specific polymorphisms were identified. From these observations, we adopted a method based on the Roger’s genetic distance between populations using the observed frequency of bands to discriminate populations pairwise. Except for one case, the between-population distances were all significantly different from zero. We have also determined the minimal number of bands and individuals required to test for the significance of between-population distances.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050756
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Insight on segregation distortions in two intraspecific crosses between annual species of Medicago (Leguminosae) (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 682-691 
    Details
    ISSN: 1432-2242
    Keywords: Key words Medicago truncatula ; Medicago tornata ; RAPD ; Segregation distortion ; DNA content
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  About 40% (α=0.05) of the PCR-derived markers scored in a Medicago truncatula and M. tornata intraspecific cross departed from Mendelian expectations at α=0.05. This proportion is among the highest ever documented in the literature, notably for intraspecific crosses. Estimations of DNA amount were also implemented for the parental genotypes or parental lines, and significant variations were observed. Our results suggest that the parental genotypes have diverged for quite a while, and we propose that the level of distortion we documented is correlated with the genome size difference we measured.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050466
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    Paper (German National Licenses)
    Fulltext
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