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  • 1
    Electronic Resource
    Electronic Resource
    Detection of somaclonal variation in garlic (Allium sativum L.) using RAPD and cytological analysis (1999)
    Springer
    Plant cell reports 18 (1999), S. 473-477 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsAllium sativum ; Garlic ; Genetic instability ; RAPD ; Somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plants were regenerated by somatic embryogenesis from long-term callus cultures derived from five garlic (Allium sativum L.) cultivars. Thirty-five of these plants were subjected to RAPD analysis. The frequency of variation was found to be cultivar dependent: approximately 1% in the two clones Solent White and California Late and around 0.35% in another three clones, Chinese, Long Keeper and Madena. Certain band changes were found in regenerants of different cultivars, suggesting the existence of a mutation-sensitive part of the garlic genome. The karyotypes of another 75 regenerants derived from the same callus cultures of three parental garlic clones were examined. Of these plants, 9.3% were found to be tetraploids, 4% aneuploid and 2.6% showed a change in the position of the secondary constriction. No association could be shown between the rate of variation for molecular and cytological characters either by comparing cultivars or examining individual regenerants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050606
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Numerical analyses of RAPD data highlight the origin of cultivated tagasaste (Chamaecytisus proliferus ssp. palmensis) in the Canary Islands (1993)
    Springer
    Theoretical and applied genetics 87 (1993), S. 264-270 
    Details
    ISSN: 1432-2242
    Keywords: Chamaecytisus ; Tagasaste ; Fodder legumes ; Multivariate analysis ; RAPD ; Plant genetic resources ; Biodiversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Random Amplified Polymorphic DNA (RAPD) was used to generate molecular markers to trace the origin of the fodder legume tagasaste (Chamaecytisus proliferus (L. fil.) Link ssp. palmensis (H. Christ) Kunkel) in the Canary Islands. Results from multivariate analyses of data through “Two Way Indicator Species Analysis” (TWINSPAN) and “Detrended Correspondence Analysis” (DECORANA) showed that genotypes collected on the island of La Palma exhibited a wider range of variation than those from the other islands. This supports the existing hypothesis that tagasaste originated on La Palma and emphasizes the importance of conserving and evaluating germ plasm from this island.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223775
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The identification of duplicate accessions within a rice germplasm collection using RAPD analysis (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 1049-1055 
    Details
    ISSN: 1432-2242
    Keywords: Oryza sativa ; Rice ; Genetic resources ; RAPD ; Molecular markers ; Cluster analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A set of accessions of Oryza sativa from the International Rice Research Institute (Philippines) that included known and suspected duplicates as well as closely related germplasm has been subjected to RAPD analysis. The number of primers, the number of polymorphic bands and the total number of bands were determined that will allow the accurate discrimination of these categories of accessions, including the identification of true and suspected duplicates. Two procedures have been described that could be employed on a more general basis for identifying duplicates in genetic resources collections, and further discussion on the values of such activities is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222920
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    The taxonomic characterisation of annual Beta germplasm in a genetic resources collection using RAPD markers (1996)
    Springer
    Euphytica 91 (1996), S. 205-212 
    Details
    ISSN: 1573-5060
    Keywords: Beta ; genetic resources ; RAPD ; annual beets ; molecular marker ; polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Annual beets in the genus Beta section Beta represent an important genetic resource. Representative accessions of annual beets from a beet germplasm collection were analysed using RAPD to assess the patterns of variation and relationships among them. Using arbitrary primers, markers showing variation across accessions were identified. A dendrogram of similarity was produced using these molecular markers. All the accessions analysed were classified into three major groups corresponding to species or subspecies macrocarpa, adanensis and maritima. Macrocarpa was shown to be the most divergent group in this section. Using RAPD molecular markers, it was possible to ascribe an accession to one of three taxonomic groups and overcome much of the confusion encountered when morphological traits are used for identification. The group of maritima was found to be more polymorphic than either the group of macrocarpa or adanensis at both accession and subspecies levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021071
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    The use of RAPD for assessing variation in plants (1993)
    Springer
    Plant growth regulation 12 (1993), S. 43-51 
    Details
    ISSN: 1573-5087
    Keywords: RAPD ; fingerprinting ; polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00144581
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    Paper (German National Licenses)
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