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  • 1
    Electronic Resource
    Electronic Resource
    Use of restriction fragment length polymorphism to fingerprint beets at the genotype and species levels (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 847-851 
    Details
    ISSN: 1432-2242
    Keywords: Sugar beet ; Beta nana ; Beta vulgaris ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary cDNA probes have been used to assess genetic variation in beet using hybridisation techniques that detect restriction fragment length polymorphism. Probes have been identified which differ in the levels of variation that they can detect (i) within closely related genetic material of sugar beet, and (ii) between sugar beet and a taxonomically distant Beta species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266669
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  • 2
    Electronic Resource
    Electronic Resource
    Determination of genetic stability using isozymes and RFLPs in beet plants regenerated in vitro (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 113-117 
    Details
    ISSN: 1432-2242
    Keywords: Beet ; Somaclonal variation ; Isozyme ; RFLP ; Genetic stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Sugar, fodder and garden beet (Beta vulgaris L.) plants have been regenerated in culture from a range of expiant material. Of the regenerants 764 were subjected to isozyme analysis using eight enzyme-specific stains, and 60 were subjected to RFLP analysis using three cDNA probes. Both molecular techniques allowed the identification of somaclonal variant plants. Assessment of the numbers of variant isozymes and restriction fragments has allowed the calculation of the approximate percentage of variant alleles occurring in any one somaclonal regenerant, namely between 0.05% and 0.1%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223989
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Comparative Analysis of Crossover Exchanges and Chiasmata in Allium Cepa × Fistulosum After Genomic In Situ Hybridization (GISH) (1998)
    Springer
    Chromosome research 6 (1998), S. 567-574 
    Details
    ISSN: 1573-6849
    Keywords: Allium ; chiasmata ; crossing over ; genomic in situ hybridization ; recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genomic in situ hybridization (GISH) successfully differentiated homoeologous genomes in the interspecific hybrid Allium cepa × fistulosum, thus allowing the detection of reciprocal crossover events as label exchanges in separating anaphase I chromosomes. Three of the eight chromosome pairs were positively identified by fluorescence in situ hybridization (FISH) to rDNA sequences. There was a general similarity of the GISH-based label exchange frequencies and metaphase I chiasma frequencies, but with a 20% deficit of chiasmata. Reasons for this apparent deficit are discussed. The locations of chiasmata and label exchanges are in broad agreement.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009296826942
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Distribution of a 375 bp repeat sequence inAllium (Alliaceae) as revealed by FISH (1999)
    Springer
    Plant systematics and evolution 217 (1999), S. 31-42 
    Details
    ISSN: 1615-6110
    Keywords: Alliaceae ; Allium ; Fluorescent in-situ hybridisation ; cytotaxonomy ; telomeric repeat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Information about evolutionary relationships between species of the genusAllium is desirable in order to facilitate breeding programmes. One approach is to study the distribution of repetitive DNA sequences among species thought on taxonomic grounds, to be closely related. We have used fluorescent in-situ hybridisation (FISH) to examine seven species within sect.Cepa of the genus (A. altaicum, A. cepa, A. fistulosum, A. galanthum, A. pskemense, A. oschaninii andA. vavilovii), one species from sect.Rhizirideum (A. roylei), two species from sect.Allium (A. sativum andA. porrum) and one species from sect.Schoenoprasum (A. schoenoprasum). Each species was probed using a 375 bp repeat sequence isolated fromA. cepa (Barnes & al. 1985), which was generated and labelled by polymerase chain reaction (PCR). No signals were detected in anyAllium species not belonging to sect.Cepa with the exception ofA. roylei, whose designation in sect.Rhizirideum is now questioned. Within sect.Cepa the probe was found to hybridize to the ‘terminal’ regions of the chromosome arms of all the species examined. In addition a number of interstitial bands were detected. Use of FISH reveals a more detailed map of the location of the repeat sequences than has previously been obtained by C-banding and other staining procedures. The distribution of the terminal and interstitial sites when compared, allow us to identify three species groups namely,A. altaicum andA. fistulosum; A. cepa, A. roylei, A. oschaninii andA. vavilovii; andA. galanthum andA. pskemense.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00984920
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    Paper (German National Licenses)
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