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  • 1
    Electronic Resource
    Electronic Resource
    cDNA cloning of a novel human gene NAKAP95, neighbor of A-kinase anchoring protein 95 (AKAP95) on chromosome 19p13.11–p13.12 region (2000)
    Springer
    Journal of human genetics 45 (2000), S. 31-37 
    Details
    ISSN: 1435-232X
    Keywords: Key words Cyclic AMP-dependent protein kinase (PKA) ; A-kinase anchoring proteins (AKAPs) ; AKAP95 ; Chromosome 19p13.11–p13.12 ; RH mapping ; Genomic structure ; Gene duplication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A-kinase anchoring protein 95 (AKAP95) is a nuclear protein which binds to the regulatory subunit (RII) of cyclic adenosine monophosphate (cAMP)-dependent protein kinase (PKA) and to DNA. A novel nuclear human gene which shares sequence homology with the human AKAP95 gene was identified by a nuclear transportation trap method. By polymerase chain reaction (PCR)-based analysis with both a human/rodent monochromosomal hybrid cell panel and a radiation hybrid panel, the gene was mapped to the chromosome 19p13.11–p13.12 region between markers WI-4669 and CHLC.GATA27C12. Furthermore, alignment with genomic sequences revealed that the gene and human AKAP95 resided tandemly only approximately 250 bp apart from each other. We designated this gene as neighbor of AKAP95 (NAKAP95). The exon-intron structure of NAKAP95 and AKAP95 was conserved, indicating that they may have evolved by gene duplication. The predicted protein product of the NAKAP95 gene consists of 646 amino acid residues, and NAKAP95 and AKAP95 had an overall 40% similarity, both having a potential nuclear localizing signal and two C2H2 type zinc finger motifs. The putative RII binding motif in AKAP95 was not conserved in NAKAP95. A reverse transcription coupled (RT)-PCR experiment revealed that the NAKAP95 gene was transcribed ubiquitously in various human tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100380070040
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation, tissue expression, and chromosomal assignment of a novel human gene which encodes a protein with RING finger motif (1998)
    Springer
    Journal of human genetics 43 (1998), S. 272-274 
    Details
    ISSN: 1435-232X
    Keywords: Key words RING finger ; Full-Length enriched cDNA library ; Chromosome 6p21.3 ; RH mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We identified a novel gene encoding a RING finger (C3HC4-type zinc finger) protein from a human neuroblastoma full-length enriched cDNA library. This cDNA clone consists of 1919 nucleotides with an open reading frame of a 485-amino acid protein. From reverse transcription (RT)-polymerase chain reaction (PCR) analysis, the messenger RNA was ubiquitously expressed in various human adult tissues. The chromosomal location of the gene was determined on the chromosome 6p21.3 region by PCR-based analyses with both a human/rodent monochromosomal hybrid cell panel and a radiation hybrid mapping panel.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100380050088
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  • 3
    Electronic Resource
    Electronic Resource
    cDNA cloning, expression profile, and genomic structure of human and mouse RNF10/Rnf 10 genes, encoding a novel RING finger protein (2000)
    Springer
    Journal of human genetics 45 (2000), S. 38-42 
    Details
    ISSN: 1435-232X
    Keywords: Key words RING finger (C3HC4-type zinc finger) motif ; RNF10 ; Rnf10 ; KIAA0262 ; RH mapping ; 12q23–q24.1 ; D5Mit318
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract RING finger (C3HC4-type zinc finger) is a variant zinc finger motif present in a new family of proteins including transcription regulators. A new member of the RING finger protein family was identified through a mouse expressed sequence tag (EST) database search, and its full-length cDNA was isolated from a mouse brain full length-enriched cDNA library. The gene was designated as Rnf10, for RING finger protein 10. The cDNA clone consists of 3110 nucleotides and encodes an open reading frame (ORF) of an 804-amino acid protein. A database search revealed that human KIAA0262 protein (accession number, D87451) has strong homology to mouse Rnf10. To confirm that mouse Rnf10 is the homolog or an isolog of human KIAA0262, a human RNF10 cDNA was cloned in our hands from a fetal brain cDNA pool. The newly isolated cDNA contained an ORF for 811 amino acids which had almost identical structure to mouse Rnf10 protein, indicating that the human ORF codes for RNF10 protein. This finding was also supported by comparative chromosome mapping in which both genes were localized in a conserved linkage homology region between mouse and human. Comparison of the RNF10 and KIAA0262 proteins revealed that both were transcribed from the same gene and that the longer RNF10 ORF would be the authentic form. The complete genomic organization of RNF10 was determined to consist of 17 exons spanning at least 40 kb in the genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100380050007
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  • 4
    Electronic Resource
    Electronic Resource
    cDNA cloning of a human RAB26-related gene encoding a Ras-like GTP-binding protein on chromosome 16p13.3 region (2000)
    Springer
    Journal of human genetics 45 (2000), S. 309-314 
    Details
    ISSN: 1435-232X
    Keywords: Key words Ras superfamily of small GTP-binding proteins ; RAB26-related ; Rab26 ; RT-PCR ; RH mapping ; Chromosome 16p13.3 ; Virtual transcribed sequence (VTS)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Members of the RAB protein family are important regulators of vesicular fusion and trafficking. A putative new member of the RAB family of genes was identified through a public database search, and its full-length cDNA was isolated from a human fetal brain cDNA library. The predicted protein product of the gene consists of 190 amino acid residues and has 87% identity with rat Rab26. Thus, we designated this gene as the human RAB26-related gene. Reverse transcription-coupled polymerase chain reaction (RT-PCR) demonstrated that the RAB26-related messenger RNA was predominantly expressed in adult and fetal brain. Furthermore, an RT-PCR experiment for brain subregions showed that the mRNA was highly expressed in the amygdala, cerebellum, caudate nucleus, and hippocampus. By PCR-based analysis with both a human/rodent monochromosomal hybrid cell panel and a radiation hybrid panel, the gene was mapped to the chromosome 16p13.3 region between markers WI-7742 and WI-3061. The RAB26-related gene consists of eight exons that span about 44 kb of the genome DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100380070023
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  • 5
    Electronic Resource
    Electronic Resource
    cDNA cloning, tissue expression, and chromosome mapping of human homolog of SOX18 (2000)
    Springer
    Journal of human genetics 45 (2000), S. 192-195 
    Details
    ISSN: 1435-232X
    Keywords: Key wordsSOX18 ; HMG-box ; RH mapping ; Chromosome 20q13.33
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The SRY (sex-determining region Y) gene encodes a transcription factor characterized by a DNA-binding motif termed the HMG (high mobility group) domain. The SOX (Sry-box) genes comprise a large family related by homology to the HMG-box region. We isolated a cDNA clone with an open reading frame encoding a putative protein of 384 amino acids, which shared 83% identity to the mouse Sox18 protein. Northern blot analysis revealed that a 1.9-kb band of human SOX18 messenger RNAs was predominantly expressed in heart, although weak signals were seen in brain, liver, testis, and leukocyte. By polymerase chain reaction (PCR)-based analyses with both a human/rodent monochromosomal hybrid cell panel and a radiation hybrid panel, the gene was mapped to the chromosome 20q13.33 region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100380050210
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