ISSN:
1615-6102
Keywords:
Chironomus
;
Polytene chromosomes
;
RNA polymerase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A method is described that allows the cytological detection of the transcriptional activity of endogenous RNA polymerase in fixedChironomus polytene chromosomes. 3H-UTP is preferentially incorporated onto nucleoli and Balbiani rings where transcription is specially high. The former incorporation was sensitive to actinomycin D while incorporation on Balbiani rings was sensitive to α-amanitin. The label pattern and frequency resemble those detected by3H-uridine incorporation in the living state.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01293437
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