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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 101 (1979), S. 81-87 
    ISSN: 1615-6102
    Keywords: Chironomus ; Polytene chromosomes ; RNA polymerase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A method is described that allows the cytological detection of the transcriptional activity of endogenous RNA polymerase in fixedChironomus polytene chromosomes. 3H-UTP is preferentially incorporated onto nucleoli and Balbiani rings where transcription is specially high. The former incorporation was sensitive to actinomycin D while incorporation on Balbiani rings was sensitive to α-amanitin. The label pattern and frequency resemble those detected by3H-uridine incorporation in the living state.
    Type of Medium: Electronic Resource
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