ISSN:
1615-6102
Keywords:
Actin circles
;
Corn endosperm
;
Fluorescein-S1-myosin
;
Protein bodies
;
Rhodamine-phalloidin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary When corn endosperm is gently homogenized in a microfilament-stabilizing buffer and filtrates centrifuged at very low g forces, abundant protein bodies are present in the pellet. When stained with both rhodamine-phalloidin and fluorescein-S1-myosin and viewed under epifluorescence, individual protein bodies appear to be enclosed in F-actin and groups of protein bodies appear to be enmeshed within a network of fine actin filaments. The yield of actinenclosed protein bodies is not affected by the addition of non-ionic detergents to the grinding medium, but it is severely decreased with the addition of cytoskeleton-disrupting agents including Tris-HCl, KCl, KI, and ammonium sulphate, and the interconnecting fine filaments are totally abolished. Heparin, which interacts with membrane-cytoskeleton complexes, causes apparent coagulation of the protein bodies. The evidence from fluorescent microscopy suggesting that F-actin surrounds and enmeshes the protein bodies is supported by electron microscopy, gel electrophoresis, and Western blot analysis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01322284
Permalink
