ISSN:
1573-9058
Keywords:
Anabaena variabilis
;
cyanobacteria
;
fluorescence microscope photographs
;
orientation
;
polarized spectra
;
polyvinyl alcohol film
;
purple bacteria
;
radiation scattering
;
Rhodospirillum rubrum
;
Synechococcus
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The photosynthetic bacteria (Rhodospirillum rubrum, Synechococcus and Anabaena variabilis) as well as their fragments embedded in isotropic and anisotropic polymer film were investigated. The orientation of photosynthetic pigments inside these organisms was compared, on the basis of the polarised absorption and fluorescence spectra, with the macroscopic orientation of investigated objects seen under microscope. The anisotropy of fluorescence was much higher than anisotropy of absorption. It showed strong influence of the photoselection by polarised radiation on the various bacterial chromophores exhibiting different orientations in the cells and various yields of fluorescence. The dimensions of cells were investigated on the basis of their photographs and by the scattering of the monochromatic radiation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1007278100996
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