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Digitale Medien

Identification and germline transformation of the ribosomal protein rp21 gene of Drosophila: Complementation analysis with the Minute QIII locus reveals nonidentity (1988)

Kay, Mark A. ; Zhang, J. -Y. ; Jacobs-Lorena, Marcelo

Springer

Molecular genetics and genomics 213 (1988), S. 354-358

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ISSN: 1617-4623

Schlagwort(e): Drosophila ; Minute ; Ribosomal protein ; Transformation

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Summary Minute loci represent a class of about 50 different Drosophila genes that appear to be functionally related. These genes may code for components of the protein synthetic apparatus. While one Minute locus has been recently shown to code for a ribosomal protein, it is not yet known whether any of the other Minute loci also code for ribosomal proteins. We have addressed this question by a combined molecular and genetic approach. In this report, a cloned DNA encoding the ribosomal protein rp21 is partially characterized. The rp21 gene maps to the same region (region 80 of chromosome 3L) as the temperature-sensitive Minute QIII gene. Using P-element mediated transformation, the rp21 gene was transformed into the germline of Drosophila. RNA blot experiments revealed that the transformed gene is expressed in transgenic flies. However, genetic complementation analysis indicated that the QIII locus and the rp21 gene are not identical. Implications of these findings for the relationship between Minutes and ribosomal protein genes are discussed.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00339602

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Digitale Medien

Chemical modification in mass spectrometry IV - 2-alkenyl-4,4-dimethyloxazolines as derivatives for the double bond location of long-chain olefinic acids (1988)

Zhang, J. Y. ; Yu, Q. T. ; Liu, B. N. ; [weitere]

Chichester : Wiley-Blackwell

Biological Mass Spectrometry 15 (1988), S. 33-44

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ISSN: 0887-6134

Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: Long-chain unsaturated fatty acids (UFA) can easily be converted on a microgram scale to the corresponding 2-alkenyl-4,4-dimethyloxazolines by condensation with 2-amino-2-methylpropanol (AMP). These modified molecules with a ‘hidden’ carboxyl group have been proved to be a class of useful derivatives for gas chromatography/mass spectrometry (GC/MS) of UFA mixtures. While possessing very good GC characteristics, the title compounds show regular, well-recognizable diagnostic ion peaks of the double bond position in the chain. Detailed description of the method as well as electron impact (E1) mass spectra of derivatives resulting from mono-, di and polyenoic (maximally containing six double bonds) acids are presented.

Zusätzliches Material: 5 Ill.