ISSN:
1432-2242
Keywords:
Semidwarfism
;
Restriction fragment length polymorphism
;
Rice (Oryza sativa L.)
;
DNA markers
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A new rice semidwarfing gene which is not allelic tosd1, temporarily designated assdg, might be of use as a new source of semidwarfism in rice breeding programs. We report here the identification of a DNA marker closely linked to this gene. The DNA marker was identified by testing 120 mapped rice RFLP makers as hybridization probes for Southern analysis of a pair of nearly isogenic lines with or withoutsdg. Linkage association of the marker with the gene was verified using a F2 population segregating for semidwarfism. RFLP analysis showed thatsdg is closely linked to a single-copy DNA clone RZ182 on chromosome 5, with a distance of 4.3 centiMorgans between them. This marker may facilitate early selection for the semidwarfing gene in rice breeding programs
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01254003
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