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  • Key wordsNeurospora crassa  (1)
  • Rye grass  (1)
  • 1
    ISSN: 1617-4623
    Keywords: Key wordsNeurospora crassa ; Actin ; Actin-related protein 3 (ARP3) ; cot-1 ; mcb
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genes encoding actin and ARP3 in the filamentous fungus Neurospora crassa were cloned and sequenced. The actin structural gene is interrupted by four introns and encodes a polypeptide of 375 amino acids, which shows very high degree of identity with actin from other sources. N. crassa ARP3 is 439 amino acids in length and is 71% to 80% identical to ARP3s from five other organisms, while actin is 40% to 50% identical to these same ARP3s. Transcript levels for actin and ARP3 decrease upon induction of asexual development (i.e. conidiation) and subsequently increase slightly when conidia are being formed. A concentration of filamentous actin is typically seen at sites of growth in eukaryotic organisms and, using indirect immunofluorescence, we showed that filamentous actin is localized primarily to hyphal tips in N. crassa. To determine if the level of actin increases in response to an increase in the number of growth sites and in the area of the growing surface, we used the temperature-sensitive mutants cot-1 and mcb. Growth of the cot-1 and mcb mutants at restrictive temperature induces hyphal tip formation and a loss of growth polarity, respectively. Unexpectedly, almost no increase in actin levels is observed following a 〉20-fold increase in the number of hyphal tips or an increase in the area of the growing surface resulting from a loss of growth polarity. The results suggest that the level of actin monomers within N. crassa hyphae is sufficient to accommodate the need for additional actin patches and filaments that arises when the number of hyphal tips and the area of growing surface per unit length of hypha greatly exceeds that in wild-type.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 55 (1980), S. 139-144 
    ISSN: 1573-5036
    Keywords: Iron ; Manganese ; Nickel toxicity ; Rye grass ; Zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Rye grass (Lolium perenne, cv.S-23) was grown for 4 weeks in a non-calcareous Seaton loam soil with varying amounts of Ni as NiSO4. The purpose of this investigation was to study the Ni toxicity and the relationship of Ni with other essential elements. Nickel depressed shoot yield at all levels except at the lowest levelviz 30 μg Ni/g soil. Nickel concentration of 50 μg/g in shoots did not reduce the dry matter production in rye grass although slight chlorosis did appear at this level. The Ni and Fe concentration of the shoots increased and that of Mn and Zn decreased with increasing rates of Ni application. Uptake of Mn and Zn decreased at all level of Ni. But Fe uptake showed a slight increase at the first two levels and a profound depression at the subsequent levels. The pattern of Ni uptake is different, being highest at the middle level and decreasing on both sides which showed that the increase of Ni concentration of shoots is not proportional to the reduction in the yield. The Ni−Fe ratio rather than Ni and Fe concentration in plants has shown better relationship with the toxic effects of Ni. The implications of Ni phytotoxicity are discussed with particular reference to serpentine soils.
    Type of Medium: Electronic Resource
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