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  • 1970-1974  (2)
  • Concussion  (1)
  • SH Reagents  (1)
Materialart
Erscheinungszeitraum
  • 1970-1974  (2)
Jahr
  • 1
    ISSN: 1432-2013
    Schlagwort(e): Proximal Kidney Tubule ; Mercurials ; SH Reagents ; Site Group Reagents ; Transtubular Transport
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The effects of site group reagents were tested on the following transport processes of the proximal convolution. Isotonic Na+ absorption, evaluated by the shrinking droplet procedure, histidine and glucose transport, evaluated by measuring the respective transtubular concentration difference at zero substance and water net flux. The test substances were applied either by continuous microperfusion of the peritubular capillaries or by luminal perfusion prior to the transport tests or by addition to the luminal test solution. The SH reagents (0.2 mM) N-ethylmaleimide,p-chloromercuribenzoate (pCMB) 3,6-bis-(acetatomercurimethyl)dioxane and Mersalyl (Salyrgane) caused 50% inhibition of the isotonic Na+ absorption in approximately 1.5 min when applied to the capillary perfusate. The same effect was reached in 2–3 min by 0.2 mMp-chloromercuriphenylsulfonate, benzamido-4-iodo-acetylstilbene-2,5-disulfonate and 2,2′-dihydroperoxy-2,2′-dibutylperoxide. However, the large molecular SH reagentspCMB-dextran T10 and benzoxanthene-3,4-dicarboxylic-N-iodoacetyloligoprolyl-2-aminoethylimid, did not inhibit the isotonic Na+ absorption. If an inhibitory effect was observed on the Na+ transport its onset was faster, when the substance was applied from the blood site than when it was given from the tubular lumen. Because SH reagents inhibit the isotonic Na transport faster when applied from the blood side, and because SH reagents with MW up to 690 are inhibitory whereas larger ones with MW over 1700 are not, it seems that they exert their inhibitory action on SH groups located a) predominantly on the blood side and b) deep within the membrane and not at the surface. Histidine- and glucose transport was inhibited only when the sodium transport was inhibited considerably. The oxygen consumption of teased kidney slices is not inhibited by 0.2 mMpCMB or Mersalyl within 10 min, but it is inhibited considerably by 1 mM of these substances in the same period of incubation time. The COOH reagents N,N′-carbonyl-diimidazole and N-ethyl-N′-(3-dimethyl-aminopropyl)carbodiimid (10 mM) and the NH2 reagents 4-acetamido-4′-isothiocyanatostilbene-2,2′-disulfonic acid, 2 Na+ (SITS) (1 mM) as well as danslychloride (applied from the lumen at 5 mM in paraffin oil) did not inhibit the isotonic Na+ absorption.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Journal of neurology 200 (1971), S. 201-212 
    ISSN: 1432-1459
    Schlagwort(e): Concussion ; Experimental Transient Unconsciousness ; Traumatic Perivenous Edema
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Ein neues Modell zur Erzeugung einer Hirnerschütterung im Tierexperiment, vergleichbar mit den Störungen beim menschlichen Trauma, wird beschrieben. Eine modifizierte Remington-Humane-„Kanone“ wurde benutzt, um einen Einzelschlag auf den Kopf eines Experimentaltieres zu richten, das in einer Weste hängend, Kopf nach oben, gehalten wurde. Von 39 Katzen starben 9 direkt nach dem experimentellen Trauma, 2 erlitten ein prolongiertes Koma. Bei den übrigen trat eine vorübergehende Bewußtlosigkeit von 10 sec bis 18 min ein, und sie überlebten, bis sie getötet wurden. Bei der histologischen Untersuchung fand sich bei einer reinen Hirnerschütterung kein perivenöses Ödem, sondern nur nach Hirnkontusion. Der Vergleichswert dieser Experimente mit den Vorgängen beim Menschen wird diskutiert.
    Notizen: Summary A model method to produce, experimentally, concussion in the cat was described and was comparable to concussion in man. A modified Remington Humane Stunner was used to apply a single blow to the head of the animal, which was suspended in a head — up position by means of a specially designed vest hanging down from the ceiling. Out of 39 cats subjected to trauma, 9 died shortly after the experiment and 2 suffered from prolonged loss of consciousness. The remaining cats showed transient loss of consciousness with full recovery from 10 sec up to 18 min later and survived until they were sacrified. The morphological study with conventional techniques revealed that perivenous edema did not occur in pure concussion, although if contusion of the brain occurred, perivenous edema existed. The comparability to the pathogenesis of perivenous edema in man was discussed.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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