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  • Cell & Developmental Biology  (5)
  • Electromyography  (5)
  • Salmonella  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Characterization of Salmonella Dublin and Salmonella Typhimurium (Copenhagen) Isolates from Cattle (1997)
    Springer
    Veterinary research communications 21 (1997), S. 409-420 
    Details
    ISSN: 1573-7446
    Keywords: aerobactin ; antibiotic ; capsule ; cattle ; colicin ; haemolysin ; plasmid ; Salmonella ; spvC gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Brackelsberg, C.A., Nolan, L.K. and Brown, J., 1997. Characterization of Salmonella dublin and Salmonella typhimurium (Copenhagen) isolates from cattle. Veterinary Research Communications, 21 (6), 409-420 Eight Salmonella typhimurium (Copenhagen) and eight Salmonella dublin isolates from cattle were compared by their antibiotic resistance patterns, by their production of colicin, aerobactin, haemolysin and capsule, by their possession of transmissible R plasmids and the spvC gene, and by their ability to invade and replicate within cultured epithelial cells. The two groups differed in their antibiotic resistance profiles, with more of the host-adapted S. dublin isolates resistant to tetracycline than were the non-host-adapted S. typhimurium (Copenhagen) group, but more of the S. typhimurium (Copenhagen) isolates resistant to the other antibiotics tested. None of the isolates produced colicin, but all produced aerobactin. One isolate in each group was encapsulated. All of the S. typhimurium (Copenhagen) and S. dublin isolates contained plasmids, and all of them contained the spvC-homologous sequences. Four of the S. typhimurium (Copenhagen) isolates were able to transfer an R plasmid to a recipient organism by conjugation. One of the five S. dublin isolates, which showed resistance to some of the antibiotics tested, was able to transfer an R plasmid by conjugation. Both groups of isolates invaded cultured epithelial cells to a similar degree after 1 h, but the S. dublin isolates reached significantly higher levels within the cells than did S. typhimurium (Copenhagen) after 9 h. This ability may, in part, explain the association of S. dublin with more severe forms of salmonellosis and prolonged carrier states. Further study of the intracellular growth of these isolates seems warranted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005803301827
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  • 2
    Electronic Resource
    Electronic Resource
    The distribution of invA, pagC and spvC genes amongSalmonella isolates from animals (1995)
    Springer
    Veterinary research communications 19 (1995), S. 167-177 
    Details
    ISSN: 1573-7446
    Keywords: diagnosis ; gene ; invA ; pagC ; Salmonella ; spvC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract New molecular diagnostic techniques often rely on hybridization or amplification of specific DNA regions to detect pathogenic bacteria. The choice of genes to be used as probes or as the targets of amplification techniques is critical to the success of these procedures. The genes so used might best be those associated with virulent isolates and having a wide distribution among such isolates. In this study three genes,invA, pagC andspvC, thought to be associated with the virulence of salmonellae, were labelled and used to probe the total DNA from 103Salmonella isolates from animals in an attempt to determine whether these genes might be useful in diagnostic procedures.pagC was detected in 99% of theSalmonella tested, andinvA was detected in 94.2% of the isolates. BothpagC andinvA were detected with a significantly higher frequency thanspvC in isolates from chickens and swine, but no significant difference in detection of these three genes occurred when bovine isolates were examined. Failure to detect any of these genes occurred in only one isolate. Isolates from apparently healthy or from clinically ill chickens and swine could not be distinguished by detecting these three genes. The genes were not detected in the non-Salmonella strains tested. These results suggest that, of these three genes,pagC may be the best choice for use as a probe or polymerase chain reaction target in future detection protocols.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01839295
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  • 3
    Electronic Resource
    Electronic Resource
    Detection and characterization ofSalmonella typhimurium from a dairy herd in North Dakota (1995)
    Springer
    Veterinary research communications 19 (1995), S. 3-8 
    Details
    ISSN: 1573-7446
    Keywords: antibiotic resistance ; cattle ; delayed secondary enrichment ; detection ; faeces ; primary enrichment ; Salmonella
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Nasal secretions, faecal samples and buffy coats were obtained from 102 cattle from a North Dakota dairy herd with a history of calf scours. Treated buffy coats, faecal samples and nasal secretions were inoculated into tetrathionate broth (TB), incubated at 37°C overnight, and plated onto brilliant green agar medium with novobiocin (BGAN). The TB was left at room temperature for 5 days and then used to inoculate fresh TB. The fresh TB was incubated at 37°C over night and plated onto BGAN medium. All the plates were incubated at 37°C over night and observed forSalmonella-like growth. Suspect colonies were further tested andSalmonella isolates were serotyped by the National Veterinary Services laboratory. Twenty-two of the 36 calves sampled harbouredS. typhimurium in their faeces, but no samples from cows were positive. NoSalmonella were isolated from the buffy coats, but 4 calves were shown to haveSalmonella in their nasal secretions. Extended enrichment of the faecal cultures in TB resulted in a significant increase inSalmonella isolations, although 2 samples were positive following the initial enrichment period and not after secondary enrichment. The typicalSalmonella isolate detected from this herd contained a transmissible R-plasmid encoding resistance to tetracycline, kanamycin, sulphisoxazole and ampicillin. This study confirmed that delayed secondary enrichment in TB is superior to primary enrichment for detection ofSalmonella from cattle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01839246
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  • 4
    Electronic Resource
    Electronic Resource
    Influence of muscle activation dynamics on reaction time in the elderly (1994)
    Springer
    European journal of applied physiology 69 (1994), S. 344-349 
    Details
    ISSN: 1439-6327
    Keywords: Aging ; Electromyography ; Reaction time ; Motor control ; Muscle contraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The aim of this investigation was to determine whether age-related changes in the dynamics of muscle activation were, in part, responsible for longer reaction times (RT) in the elderly. A group of 12 young (mean age, 20.6 years) and 12 elderly (mean age, 64.3 years) women performed a series of ballistic forearm supination movements in response to an auditory stimulus while using a simple reaction time test. Surface electromyographic waveforms from biceps brachii (agonist) and pronator teres (antagonist) muscles were recorded, together with the angle-time curves representing the motion of the forearm, on to an IBM compatible microcomputer. The results showed that an age-related increase (P〈0.05) in motor reaction time (MRT) contributed to longer RT in the elderly. In addition, the longer (P〈0.05) MRTs in the elderly were associated with a significantly slower rate (P〈0.05) of biceps brachii muscle activation and a significantly increased proportion (P〈0.05) of the initial biceps brachii muscle burst required to initiate the movement. This data suggested that an important part of the slowing of motor behaviour, commonly observed with increasing age, may be due to either decreases in the ability of aged skeletal muscle to rapidly generate tension or to a reduction in motor drive.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392041
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  • 5
    Electronic Resource
    Electronic Resource
    Muscles within muscles: the neuromotor control of intra-muscular segments (1998)
    Springer
    European journal of applied physiology 78 (1998), S. 219-225 
    Details
    ISSN: 1439-6327
    Keywords: Key words Functional differentiation ; Electromyography ; Deltoid ; Motor control ; Muscle function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The aim of this investigation was to anatomically identify, and then determine the function of, individual segments within the human deltoid muscle. The anatomical structure of the deltoid was determined through dissection and/or observation of the shoulder girdles of 11 male cadavers (aged 65–84 years). These results indicate that the deltoid consists of seven anatomical segments (D1–D7) based upon the distinctive arrangement of each segment's origin and insertion. Radiographic analysis of a cadaveric shoulder joint suggested that only the postero-medial segment D7 has a line of action directed below the shoulder joint's axis of rotation. The functional role of each individual segment was then determined utilising an electromyographic (EMG) technique. Seven miniature (1 mm active plate; 7 mm interelectrode distance) bipolar surface electrodes were positioned over the proximal portion of each segment's muscle belly in 18 male and female subjects (18–30 years). EMG waveforms were then recorded during the production of rapid isometric shoulder abduction and adduction force impulses with the shoulder joint in 40 degrees of abduction in the plane of the scapula. Each subject randomly performed 15 abduction and 15 adduction isometric force impulses following a short familiarisation period. All subjects received visual feed back on the duration and amplitude of each isometric force impulse produced via a visual force-time display which compared subject performance to a criterion force-time curve. Movement time was 400 ms (time-to-peak isometric force) at an intensity level of 50% maximal voluntary contraction. Temporal and intensity analyses of the EMG waveforms, as well as temporal analysis of the isometric force impulses, revealed the neuromotor control strategies utilised by the CNS to control the activity of each muscle segment. The results showed that segmental neuromotor control strategies differ across the breadth of the muscle and that individual segments of the deltoid can be identified as having either “prime mover”, “synergist”, “stabiliser” or “antagonist” functions; functional classifications normally associated with whole muscle function. Therefore, it was concluded that the CNS can “fine tune” the activity of at least six discrete segments within the human deltoid muscle to efficiently meet the demands of the imposed motor task.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004210050410
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  • 6
    Electronic Resource
    Electronic Resource
    Observations on preadipocytes and their distribution patterns in rat adipose tissue (1981)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 168 (1981), S. 281-288 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Microscopic examination of adipocytes isolated from adult rat epididymal adipose tissue revealed numerous small cells (〈 10 μm) morphologically similar to larger adipocytes. These small adipocytes appear identical to a new classification of adipose cells termed preadipocytes. Electron micrographs of these preadipocytes revealed examples of cells 〈 10 μm in diameter in various stages of maturation and lipid accumulation. The percent distribution pattern of these small adipocytes was not significantly altered by exercise although exercise shifted the distribution patterns of the larger cells (〉 30 μm) toward a smaller mean cell size. The quantitative significance of preadipocytes is not established but these preliminary observations indicate that adipocytes 〈 10 μm in diameter may account for a numerically greater proportion of the total adipocytes observed in collagenase isolated preparations than heretofore recognized, although their contribution to total adipose mass is probably negligible.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051680304
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  • 7
    Electronic Resource
    Electronic Resource
    The electromyographical basis of inaccurate motor performance (1988)
    Springer
    European journal of applied physiology 58 (1988), S. 132-140 
    Details
    ISSN: 1439-6327
    Keywords: Motor control ; Motor skill ; Electromyography ; Inaccuracy ; Isometric
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The aim of this study was to investigate the electromyographical basis of inaccurate movement by identifying the variate and invariate characteristics of a ballistic, isometric precision-grip skill. Our results have shown that in most subjects investigated, IEMG, discharge rate and the paterns of muscle synergy were variate movement parameters while the sequence of muscle activation, the relative duration of muscle activity and the time to peak force (rise-time) remained essentially invariante. Based on these results, we can conclude that inaccurate performance of this skill, in most of the subjects investigated, was more dependant upon changes in muscle IEMG than in alterations in the temporal sequencing of muscle activity. It was apparent that from trial to trial, subjects had more difficulty in reproducing the appropriate levels of muscle contraction and synergy than in reproducing the required periods of action.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00636616
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  • 8
    Electronic Resource
    Electronic Resource
    Electromyographic basis of inaccurate movement; its dependence upon the mode of muscle contraction (1991)
    Springer
    European journal of applied physiology 62 (1991), S. 162-170 
    Details
    ISSN: 1439-6327
    Keywords: Motor control ; Electromyography ; Muscle contraction ; Inaccurate movement ; Motor skill
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The electromyographic basis of inaccurate performance was investigated in two rapid precision-grip skills controlled by concentric and eccentric muscle contractions respectively. Surface electromyograms, recorded from the first dorsal interosseous (DI), adductor pollicis (AP) and abductor pollicis brevis, were utilised to identify changes in the timing and intensity of muscle activation which may be responsible for inaccurate performance. The results showed that when fast precision-grip skills were controlled by concentric DI and AP muscle contractions, variations in the intensity of muscle contraction were responsible for inaccurate performance. However, when these skills were controlled by eccentric DI and AP muscle contractions, inaccurate performance resulted from variations in the timing of muscle activation. It was concluded that the nature of the deficiency in the patterns of muscle activation resulting in inaccurate performance was dependent upon the type of muscle contraction used in the skill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00643736
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  • 9
    Electronic Resource
    Electronic Resource
    Transition from slow to ballistic movement: development of triphasic electromyogram patterns (1991)
    Springer
    European journal of applied physiology 63 (1991), S. 381-386 
    Details
    ISSN: 1439-6327
    Keywords: Motor control ; Electromyography ; Motor skill ; Triphasic electromyogram ; Muscle contraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The purpose of this investigation was to determine how the triphasic electromyogram (EMG) pattern of muscle activation developed from the agonist muscle only pattern as movement time (t mov) decreased. Six adult women produced a series of 30° elbow extension movements in the horizontal plane at speeds ranging from ballistic (〈 400-ms t mov) to very slow (〉 800-ms t mov). Surface EMG from triceps brachii (agonist) and biceps brachii (antagonist) muscles were recorded, together with elbow angle, on a microcomputer. The results showed that triphasic EMG patterns developed systematically as t mov decreased from 1000 ms to 〈 200 ms. In trials with very long t mov, many elbow extension movements were produced by a single continuous activation of the agonist triceps brachii muscle. As t mov decreased however, agonist activation became predominantly burst-like and other components of the triphasic EMG pattern [activation of the antagonist (Ant) and second agonist activation (Ag2)] began to appear. At the fastest movement speeds, triphasic EMG patterns (Ag1-Ant-Ag2, Ag1 being first activation of agonist muscle) were always present. This data indicated that the triphasic pattern of muscle activation was not switched on when a particular t mov was achieved. Rather, each component systematically developed until all were present, as distinctive bursts of activity, in most trials with t mov less than 400 ms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00364466
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  • 10
    Electronic Resource
    Electronic Resource
    Renal microvasculature of the rainbow trout, Salmo gairdneri: Scanning electron microscopy of corrosion casts of glomeruli (1985)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 213 (1985), S. 505-513 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Scanning electron microscopy of corrosion casts of blood vessels permits detailed and accurate study of the microcirculation. The present study examined the renal microvasculature of the rainbow trout, Salmo gairdneri. The conventional picture of a glomerulus with one afferent arteriole was common, but glomeruli were often supplied by two afferent arterioles. In the majority of these, the intrarenal artery gave rise to a single afferent arteriole that branched to form two smaller vessels before reaching the glomerulus. Glomeruli with two afferent arterioles that arose independently from the intrarenal artery also occurred. The majority of glomeruli had a single efferent arteriole, but a proportion of glomeruli had two efferent arterioles. Efferent arterioles were smaller in diameter than the afferent arterioles. The glomerular capillaries were arranged in lobules, with few anastomoses between lobules, so that, for glomeruli with two afferent or two efferent arterioles, vascular perfusion and thus filtration within discrete lobules is probable.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092130405
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