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  • 1
    Electronic Resource
    Electronic Resource
    Distribution and evolution of two satellite DNAs in the genus Beta (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 793-799 
    Details
    ISSN: 1432-2242
    Keywords: B. vulgaris ; Wild beet ; Satellite DNA ; Genome organization ; Sequence analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary EcoRI monomers of a highly repetitive DNA family of Beta vulgaris have been cloned. Sequence analysis revealed that the repeat length varies between 157–160 bp. The percentage of AT-residues is 62% on average. The basic repeat does not show significant homology to the BamHI sequence family of B. vulgaris that was analyzed by us earlier. Both the EcoRI and BamHI sequences are investigated and compared to each other with respect to their genomic organization in the genus Beta. Both repeats were found to be tandemly arranged in the genome of B. vulgaris in a satellite-like manner. The EcoRI satellite DNA is present in three sections (Beta, Corollinae and Nanae) of the genus, whereas the BamHI satellite DNA exists only in the section Beta. The distribution of the EcoRI and BamHI satellite families in the genus is discussed with respect to their evolution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00227327
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular and physical organization of highly repetitive, undermethylated DNA from Pennisetum glaucum (1994)
    Springer
    Molecular genetics and genomics 244 (1994), S. 420-425 
    Details
    ISSN: 1617-4623
    Keywords: Pennisetum glaucum ; Satellite DNA ; In situ hybridization ; Centromeric heterochromatin ; Methylation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A HaeIIl monomer of a repetitive DNA family from Pennisetum glaucum (L.) R. Br. cv. Massue has been cloned and characterized. The repeat is 137 bp long and is organized in head-to-tail orientation in tandem arrays. The HaeIII monomer contains 55% A+T residues. The distribution of this highly repetitive sequence in different Pennisetum species and in other cereals was investigated. The HaeIII satellite is present in all Pennisetum species investigated but absent from other genera examined. In situ hybridization revealed a centromeric localization of this sequence on all seven chromosome pairs and indicated chromosome-specific differences in copy number. Methylation was investigated by comparative restriction enzyme analysis (Msp/HpaII) which showed a greater extent of methylation of the internal C of the enzyme recognition site 5′-CCGG. A South-Western analysis, using an anti-methylcytosine antibody to examine the methylation status in P. glaucum confirmed that the sequence is not highly methylated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00286694
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    Paper (German National Licenses)
    Fulltext
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